Next Article in Journal
Signal Detection and Monitoring Based on Longitudinal Healthcare Data
Next Article in Special Issue
Protein Adsorption Patterns and Analysis on IV Nanoemulsions—The Key Factor Determining the Organ Distribution
Previous Article in Journal
Dynamic Contrast-Enhanced Magnetic Resonance Imaging (DCE-MRI) in Preclinical Studies of Antivascular Treatments
Previous Article in Special Issue
Impact of Molecular Weight on Lymphatic Drainage of a Biopolymer-Based Imaging Agent
Article Menu

Export Article

Open AccessArticle
Pharmaceutics 2012, 4(4), 590-606; doi:10.3390/pharmaceutics4040590

Composition Influence on Pulmonary Delivery of Rifampicin Liposomes

Department of Environmental and Life Science, University of Cagliari, Cagliari 09124, Italy
Department of Pharmacy and Pharmaceutical Technology, University of Valencia, Valencia 46100-Burjassot, Spain
Author to whom correspondence should be addressed.
Received: 18 September 2012 / Revised: 1 November 2012 / Accepted: 16 November 2012 / Published: 27 November 2012
(This article belongs to the Special Issue Drug Delivery Using Nanotechnology)
View Full-Text   |   Download PDF [2277 KB, uploaded 27 November 2012]   |  


The effects of lipid concentration and composition on the physicochemical properties, aerosol performance and in vitro toxicity activity of several rifampicin-loaded liposomes were investigated. To this purpose, six liposome formulations containing different amounts of soy phosphatidylcholine and hydrogenated soy phosphatidylcholine, with and without cholesterol and oleic acid, were prepared and fully characterized. Uni- or oligo-lamellar, small (~100 nm), negatively charged (~60 mV) vesicles were obtained. Lipid composition affected aerosol delivery features of liposomal rifampicin; in particular, the highest phospholipid concentration led to a better packing of the vesicular bilayers with a consequent higher nebulization stability. The retention of drug in nebulized vesicles (NER%) was higher for oleic acid containing vesicles (55% ± 1.4%) than for the other samples (~47%). A549 cells were used to evaluate intracellular drug uptake and in vitro toxicity activity of rifampicin-loaded liposomes in comparison with the free drug. Cell toxicity was more evident when oleic acid containing liposomes were used.
Keywords: liposomes; rifampicin; cholesterol; oleic acid; rheology; pulmonary delivery; aerosol; cell viability; cellular uptake liposomes; rifampicin; cholesterol; oleic acid; rheology; pulmonary delivery; aerosol; cell viability; cellular uptake

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Manca, M.L.; Sinico, C.; Maccioni, A.M.; Diez, O.; Fadda, A.M.; Manconi, M. Composition Influence on Pulmonary Delivery of Rifampicin Liposomes. Pharmaceutics 2012, 4, 590-606.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Pharmaceutics EISSN 1999-4923 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top