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Pharmaceutics 2018, 10(2), 62; https://doi.org/10.3390/pharmaceutics10020062

Magnetic Nanoparticles Conjugated with Peptides Derived from Monocyte Chemoattractant Protein-1 as a Tool for Targeting Atherosclerosis

1
Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan
2
Department of Applied Chemistry, National Pingtung University, Pingtung 90003, Taiwan
3
Department and Graduate Institute of Pharmacology College of Medicine, National Taiwan University, Taipei 10617, Taiwan
4
Institute of Medical Device and Imaging, National Taiwan University, Taipei 10617, Taiwan
5
Molecular Imaging Center, National Taiwan University, Taipei 10617, Taiwan
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 26 April 2018 / Revised: 21 May 2018 / Accepted: 21 May 2018 / Published: 24 May 2018
(This article belongs to the Special Issue Functional Nanohybrids for Drug Delivery)
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Abstract

Atherosclerosis is a multifactorial inflammatory disease that may progress silently for long period, and it is also widely accepted as the main cause of cardiovascular diseases. To prevent atherosclerotic plaques from generating, imaging early molecular markers and quantifying the extent of disease progression are desired. During inflammation, circulating monocytes leave the bloodstream and migrate into incipient lipid accumulation in the artery wall, following conditioning by local growth factors and proinflammatory cytokines; therefore, monocyte accumulation in the arterial wall can be observed in fatty streaks, rupture-prone plaques, and experimental atherosclerosis. In this work, we synthesized monocyte-targeting iron oxide magnetic nanoparticles (MNPs), which were incorporated with the peptides derived from the chemokine receptor C-C chemokine receptor type 2 (CCR2)-binding motif of monocytes chemoattractant protein-1 (MCP-1) as a diagnostic tool for potential atherosclerosis. MCP-1-motif MNPs co-localized with monocytes in in vitro fluorescence imaging. In addition, with MNPs injection in ApoE knockout mice (ApoE KO mice), the well-characterized animal model of atherosclerosis, MNPs were found in specific organs or regions which had monocytes accumulation, especially the aorta of atherosclerosis model mice, through in vivo imaging system (IVIS) imaging and magnetic resonance imaging (MRI). We also performed Oil Red O staining and Prussian Blue staining to confirm the co-localization of MCP-1-motif MNPs and atherosclerosis. The results showed the promising potential of MCP-1-motif MNPs as a diagnostic agent of atherosclerosis. View Full-Text
Keywords: iron oxide magnetic nanoparticle; monocytes; MCP-1; atherosclerosis iron oxide magnetic nanoparticle; monocytes; MCP-1; atherosclerosis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Kao, C.-W.; Wu, P.-T.; Liao, M.-Y.; Chung, I.-J.; Yang, K.-C.; Tseng, W.-Y.I.; Yu, J. Magnetic Nanoparticles Conjugated with Peptides Derived from Monocyte Chemoattractant Protein-1 as a Tool for Targeting Atherosclerosis. Pharmaceutics 2018, 10, 62.

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