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Viruses 2017, 9(8), 195; doi:10.3390/v9080195

Vaccination with Recombinant Baculovirus Expressing Ranavirus Major Capsid Protein Induces Protective Immunity in Chinese Giant Salamander, Andrias davidianus

Yellow River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 2 Fenghui Road, South of Sanqiao, District of Weiyang, Xi’an 710086, China
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Author to whom correspondence should be addressed.
Academic Editor: Curt Hagedorn
Received: 13 June 2017 / Revised: 20 July 2017 / Accepted: 21 July 2017 / Published: 25 July 2017
(This article belongs to the Section Antivirals & Vaccines)
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Abstract

The Chinese giant salamander iridovirus (CGSIV), belonging to the genus Ranavirus in the family Iridoviridae, is the causative agent of an emerging infectious disease causing high mortality of more than 90% and economic losses in Chinese giant salamanders in China. In this study, a recombinant baculovirus-based vaccine expressing the CGSIV major capsid protein (MCP) was developed and its protective immunity in Chinese giant salamanders was evaluated. The recombinant Autographa californica nucleopolyhedrosis virus (AcNPV), expressing CGSIV MCP, designated as AcNPV-MCP, was generated with the highest titers of 1 × 108 plaque forming units/mL (PFU/mL) and confirmed by Western blot and indirect immunofluorescence (IIF) assays. Western blot analysis revealed that the expressed MCP reacted with mouse anti-MCP monoclonal antibodies at the band of about 53 kDa. The results of IIF indicated that the MCP was expressed in the infected Spodoptera frugiperda 9 (Sf9) cells with the recombinant baculovirus, and the Chinese giant salamander muscle cells also transduced with the AcNPV-MCP. Immunization with the recombinant baculovirus of AcNPV-MCP elicited robust specific humoral immune responses detected by ELISA and neutralization assays and potent cellular immune responses in Chinese giant salamanders. Importantly, the effective immunization conferred highly protective immunity for Chinese giant salamanders against CGSIV challenge and produced a relative percent of survival rate of 84%. Thus, the recombinant baculovirus expressing CGSIV MCP can induce significant immune responses involving both humoral and cell-mediated immunity in Chinese giant salamanders and might represent a potential baculovirus based vaccine candidate for Chinese giant salamanders against CGSIV. View Full-Text
Keywords: Chinese giant salamander; Ranavirus; major capsid protein; vaccination; protective immunity Chinese giant salamander; Ranavirus; major capsid protein; vaccination; protective immunity
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Zhou, X.; Zhang, X.; Han, Y.; Jia, Q.; Gao, H. Vaccination with Recombinant Baculovirus Expressing Ranavirus Major Capsid Protein Induces Protective Immunity in Chinese Giant Salamander, Andrias davidianus. Viruses 2017, 9, 195.

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