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Viruses 2017, 9(6), 133; doi:10.3390/v9060133

Redistribution of Endosomal Membranes to the African Swine Fever Virus Replication Site

1
Department of Biotechnology, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, INIA, Ctra. de la Coruña Km 7.5, 28040 Madrid, Spain
2
Department of Infectious Diseases, King’s College London School of Medicine, Guy’s Hospital, London SE1 9RT, UK
3
Department of Microbiology and Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA
*
Author to whom correspondence should be addressed.
Received: 4 April 2017 / Revised: 19 May 2017 / Accepted: 25 May 2017 / Published: 1 June 2017
(This article belongs to the Special Issue Porcine Viruses)
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Abstract

African swine fever virus (ASFV) infection causes endosomal reorganization. Here, we show that the virus causes endosomal congregation close to the nucleus as the infection progresses, which is necessary to build a compact viral replication organelle. ASFV enters the cell by the endosomal pathway and reaches multivesicular late endosomes. Upon uncoating and fusion, the virus should exit to the cytosol to start replication. ASFV remodels endosomal traffic and redistributes endosomal membranes to the viral replication site. Virus replication also depends on endosomal membrane phosphoinositides (PtdIns) synthesized by PIKfyve. Endosomes could act as platforms providing membranes and PtdIns, necessary for ASFV replication. Our study has revealed that ASFV reorganizes endosome dynamics, in order to ensure a productive infection. View Full-Text
Keywords: african swine fever virus (ASFV); ASF viral factory; endosomes; endosomal pathway; phosphoinositides; phosphatidylinositol kinases; microtubules african swine fever virus (ASFV); ASF viral factory; endosomes; endosomal pathway; phosphoinositides; phosphatidylinositol kinases; microtubules
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Cuesta-Geijo, M.Á.; Barrado-Gil, L.; Galindo, I.; Muñoz-Moreno, R.; Alonso, C. Redistribution of Endosomal Membranes to the African Swine Fever Virus Replication Site. Viruses 2017, 9, 133.

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