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Viruses 2017, 9(5), 109; doi:10.3390/v9050109

A Novel Roseosiphophage Isolated from the Oligotrophic South China Sea

1
State Key Laboratory of Marine Environmental Science, Institute of Marine Microbes and Ecospheres, Xiamen University (Xiang’an), Xiamen 361102, Fujian, China
2
Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editors: Mathias Middelboe and Corina P. D. Brussaard
Received: 28 February 2017 / Revised: 22 April 2017 / Accepted: 10 May 2017 / Published: 15 May 2017
(This article belongs to the Special Issue Marine Viruses 2016)
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Abstract

The Roseobacter clade is abundant and widespread in marine environments and plays an important role in oceanic biogeochemical cycling. In this present study, a lytic siphophage (labeled vB_DshS-R5C) infecting the strain type of Dinoroseobacter shibae named DFL12T, which is part of the Roseobacter clade, was isolated from the oligotrophic South China Sea. Phage R5C showed a narrow host range, short latent period and low burst size. The genome length of phage R5C was 77, 874 bp with a G+C content of 61.5%. Genomic comparisons detected no genome matches in the GenBank database and phylogenetic analysis based on DNA polymerase I revealed phylogenetic features that were distinct to other phages, suggesting the novelty of R5C. Several auxiliary metabolic genes (e.g., phoH gene, heat shock protein and queuosine biosynthesis genes) were identified in the R5C genome that may be beneficial to the host and/or offer a competitive advantage for the phage. Among siphophages infecting the Roseobacter clade (roseosiphophages), four gene transfer agent-like genes were commonly located with close proximity to structural genes, suggesting that their function may be related to the tail of siphoviruses. The isolation and characterization of R5C demonstrated the high genomic and physiological diversity of roseophages as well as improved our understanding of host–phage interactions and the ecology of the marine Roseobacter. View Full-Text
Keywords: roseophage; genome; phylogenetic analysis; environmental distribution roseophage; genome; phylogenetic analysis; environmental distribution
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Yang, Y.; Cai, L.; Ma, R.; Xu, Y.; Tong, Y.; Huang, Y.; Jiao, N.; Zhang, R. A Novel Roseosiphophage Isolated from the Oligotrophic South China Sea. Viruses 2017, 9, 109.

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