Nasal Infection of Enterovirus D68 Leading to Lower Respiratory Tract Pathogenesis in Ferrets (Mustela putorius furo)
AbstractData from EV-D68-infected patients demonstrate that pathological changes in the lower respiratory tract are principally characterized by severe respiratory illness in children and acute flaccid myelitis. However, lack of a suitable animal model for EV-D68 infection has limited the study on the pathogenesis of this critical pathogen, and the development of a vaccine. Ferrets have been widely used to evaluate respiratory virus infections. In the current study, we used EV-D68-infected ferrets as a potential animal to identify impersonal indices, involving clinical features and histopathological changes in the upper and lower respiratory tract (URT and LRT). The research results demonstrate that the EV-D68 virus leads to minimal clinical symptoms in ferrets. According to the viral load detection in the feces, nasal, and respiratory tracts, the infection and shedding of EV-D68 in the ferret model was confirmed, and these results were supported by the EV-D68 VP1 immunofluorescence confocal imaging with α2,6-linked sialic acid (SA) in lung tissues. Furthermore, we detected the inflammatory cytokine/chemokine expression level, which implied high expression levels of interleukin (IL)-1a, IL-8, IL-5, IL-12, IL-13, and IL-17a in the lungs. These data indicate that systemic observation of responses following infection with EV-D68 in ferrets could be used as a model for EV-D68 infection and pathogenesis. View Full-Text
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Zheng, H.-W.; Sun, M.; Guo, L.; Wang, J.-J.; Song, J.; Li, J.-Q.; Li, H.-Z.; Ning, R.-T.; Yang, Z.-N.; Fan, H.-T.; He, Z.-L.; Liu, L.-D. Nasal Infection of Enterovirus D68 Leading to Lower Respiratory Tract Pathogenesis in Ferrets (Mustela putorius furo). Viruses 2017, 9, 104.
Zheng H-W, Sun M, Guo L, Wang J-J, Song J, Li J-Q, Li H-Z, Ning R-T, Yang Z-N, Fan H-T, He Z-L, Liu L-D. Nasal Infection of Enterovirus D68 Leading to Lower Respiratory Tract Pathogenesis in Ferrets (Mustela putorius furo). Viruses. 2017; 9(5):104.Chicago/Turabian Style
Zheng, Hui-Wen; Sun, Ming; Guo, Lei; Wang, Jing-Jing; Song, Jie; Li, Jia-Qi; Li, Hong-Zhe; Ning, Ruo-Tong; Yang, Ze-Ning; Fan, Hai-Tao; He, Zhan-Long; Liu, Long-Ding. 2017. "Nasal Infection of Enterovirus D68 Leading to Lower Respiratory Tract Pathogenesis in Ferrets (Mustela putorius furo)." Viruses 9, no. 5: 104.