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Viruses 2017, 9(4), 67; doi:10.3390/v9040067

Identification of HIV-1 Tat-Associated Proteins Contributing to HIV-1 Transcription and Latency

1
Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, NY 14642, USA
2
Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, NY 14642, USA
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Andrew Mehle
Received: 13 February 2017 / Revised: 19 March 2017 / Accepted: 24 March 2017 / Published: 1 April 2017
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Abstract

Human immunodeficiency virus type 1 (HIV-1) Tat is a virus-encoded trans-activator that plays a central role in viral transcription. We used our recently developed parallel analysis of in vitro translated open reading frames (ORFs) (PLATO) approach to identify host proteins that associate with HIV-1 Tat. From this proteomic assay, we identify 89 Tat-associated proteins (TAPs). We combine our results with other datasets of Tat or long terminal repeat (LTR)-associated proteins. For some of these proteins (NAT10, TINP1, XRCC5, SIN3A), we confirm their strong association with Tat. These TAPs also suppress Tat-mediated HIV-1 transcription. Removing suppression of HIV-1 transcription benefits the reversal of post-integrated, latent HIV-1 proviruses. We demonstrate that these transcriptionally suppressing TAPs contribute to HIV-1 latency in Jurkat latency (J-LAT) cells. Therefore, our proteomic analysis highlights the previously unappreciated TAPs that play a role in maintaining HIV-1 latency and can be further studied as potential pharmacological targets for the “shock and kill” HIV-1 cure strategy. View Full-Text
Keywords: HIV; Tat; transcription; latency; proteomics; PLATO; protein interaction; ribosome display HIV; Tat; transcription; latency; proteomics; PLATO; protein interaction; ribosome display
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MDPI and ACS Style

Jean, M.J.; Power, D.; Kong, W.; Huang, H.; Santoso, N.; Zhu, J. Identification of HIV-1 Tat-Associated Proteins Contributing to HIV-1 Transcription and Latency. Viruses 2017, 9, 67.

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