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Viruses 2017, 9(11), 334; doi:10.3390/v9110334

Begomoviral Movement Protein Effects in Human and Plant Cells: Towards New Potential Interaction Partners

1
Department Biologie, Lehrstuhl Biochemie, Universität Erlangen-Nürnberg, Staudtstr. 5, 91058 Erlangen, Germany
2
Abteilung Molekularbiologie und Virologie der Pflanzen, Institut für Biomaterialien und Biomolekulare Systeme, Universität Stuttgart, Pfaffenwaldring 57, 70569 Stuttgart, Germany
3
Institute for Interfacial Engineering and Plasma Technology IGVP, Universität Stuttgart, Nobelstrasse 12, 70569 Stuttgart, Germany
4
Institute for Clinical and Molecular Virology, Universität Erlangen-Nürnberg, 91054 Erlangen, Germany
5
Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstr. 7 B, 38124 Braunschweig, Germany
*
Author to whom correspondence should be addressed.
Received: 9 October 2017 / Revised: 3 November 2017 / Accepted: 7 November 2017 / Published: 9 November 2017
(This article belongs to the Special Issue Geminiviruses)
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Abstract

Geminiviral single-stranded circular DNA genomes replicate in nuclei so that the progeny DNA has to cross both the nuclear envelope and the plasmodesmata for systemic spread within plant tissues. For intra- and intercellular transport, two proteins are required: a nuclear shuttle protein (NSP) and a movement protein (MP). New characteristics of ectopically produced Abutilon mosaic virus (AbMV) MP (MPAbMV), either authentically expressed or fused to a yellow fluorescent protein or epitope tags, respectively, were determined by localization studies in mammalian cell lines in comparison to plant cells. Wild-type MPAbMV and the distinct MPAbMV: reporter protein fusions appeared as curled threads throughout mammalian cells. Co-staining with cytoskeleton markers for actin, intermediate filaments, or microtubules identified these threads as re-organized microtubules. These were, however, not stabilized by the viral MP, as demonstrated by nocodazole treatment. The MP of a related bipartite New World begomovirus, Cleome leaf crumple virus (ClLCrV), resulted in the same intensified microtubule bundling, whereas that of a nanovirus did not. The C-terminal section of MPAbMV, i.e., the protein’s oligomerization domain, was dispensable for the effect. However, MP expression in plant cells did not affect the microtubules network. Since plant epidermal cells are quiescent whilst mammalian cells are proliferating, the replication-associated protein RepAbMV protein was then co-expressed with MPAbMV to induce cell progression into S-phase, thereby inducing distinct microtubule bundling without MP recruitment to the newly formed threads. Co-immunoprecipitation of MPAbMV in the presence of RepAbMV, followed by mass spectrometry identified potential novel MPAbMV-host interaction partners: the peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 (Pin4) and stomatal cytokinesis defective 2 (SCD2) proteins. Possible roles of these putative interaction partners in the begomoviral life cycle and cytoskeletal association modes are discussed. View Full-Text
Keywords: Geminiviridae; begomovirus; Abutilon mosaic virus; Cleome leaf crumple virus; microtubule; movement protein; mammalian cells; plant cells; ectopic expression; tropism; cytoskeleton Geminiviridae; begomovirus; Abutilon mosaic virus; Cleome leaf crumple virus; microtubule; movement protein; mammalian cells; plant cells; ectopic expression; tropism; cytoskeleton
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Krapp, S.; Schuy, C.; Greiner, E.; Stephan, I.; Alberter, B.; Funk, C.; Marschall, M.; Wege, C.; Bailer, S.M.; Kleinow, T.; Krenz, B. Begomoviral Movement Protein Effects in Human and Plant Cells: Towards New Potential Interaction Partners. Viruses 2017, 9, 334.

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