Next Article in Journal
HTLV-1 Infection and Adult T-Cell Leukemia/Lymphoma—A Tale of Two Proteins: Tax and HBZ
Previous Article in Journal
In Vitro Synthesized RNA Generated from cDNA Clones of Both Genomic Components of Cucurbit yellow stunting disorder virus Replicates in Cucumber Protoplasts
Article Menu

Export Article

Open AccessArticle
Viruses 2016, 8(6), 98; doi:10.3390/v8060098

Identification of Vimentin as a Potential Therapeutic Target against HIV Infection

1
Center for Genetic Engineering and Biotechnology, Ave 31/158 and 190, Cubanacán, Playa, P.O. Box 6162, Havana 10600, Cuba
2
Laboratory for AIDS Research, Carretera de Tapaste y Autopista Nacional, San José de las Lajas, Mayabeque CP 32700, Cuba
*
Author to whom correspondence should be addressed.
Academic Editor: Eric O. Freed
Received: 1 October 2015 / Revised: 1 April 2016 / Accepted: 6 April 2016 / Published: 15 June 2016
View Full-Text   |   Download PDF [4310 KB, uploaded 15 June 2016]   |  

Abstract

A combination of antiviral drugs known as antiretroviral therapy (ART) has shown effectiveness against the human immunodeficiency virus (HIV). ART has markedly decreased mortality and morbidity among HIV-infected patients, having even reduced HIV transmission. However, an important current disadvantage, resistance development, remains to be solved. Hope is focused on developing drugs against cellular targets. This strategy is expected to prevent the emergence of viral resistance. In this study, using a comparative proteomic approach in MT4 cells treated with an anti-HIV leukocyte extract, we identified vimentin, a molecule forming intermediate filaments in the cell, as a possible target against HIV infection. We demonstrated a strong reduction of an HIV-1 based lentivirus expressing the enhanced green fluorescent protein (eGFP) in vimentin knockdown cells, and a noteworthy decrease of HIV-1 capsid protein antigen (CAp24) in those cells using a multiround infectivity assay. Electron micrographs showed changes in the structure of intermediate filaments when MT4 cells were treated with an anti-HIV leukocyte extract. Changes in the structure of intermediate filaments were also observed in vimentin knockdown MT4 cells. A synthetic peptide derived from a cytoskeleton protein showed potent inhibitory activity on HIV-1 infection, and low cytotoxicity. Our data suggest that vimentin can be a suitable target to inhibit HIV-1. View Full-Text
Keywords: leukocyte extract; vimentin; intermediate filaments; cytoskeleton; HIV; anti-HIV activity; proteomics leukocyte extract; vimentin; intermediate filaments; cytoskeleton; HIV; anti-HIV activity; proteomics
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Fernández-Ortega, C.; Ramírez, A.; Casillas, D.; Paneque, T.; Ubieta, R.; Dubed, M.; Navea, L.; Castellanos-Serra, L.; Duarte, C.; Falcon, V.; Reyes, O.; Garay, H.; Silva, E.; Noa, E.; Ramos, Y.; Besada, V.; Betancourt, L. Identification of Vimentin as a Potential Therapeutic Target against HIV Infection. Viruses 2016, 8, 98.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top