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Open AccessCommunication
Viruses 2016, 8(6), 119; doi:10.3390/v8060119

Cloning the Horse RNA Polymerase I Promoter and Its Application to Studying Influenza Virus Polymerase Activity

1,2,†
,
1,2,†
,
1,2
,
1,2
,
1,2
and
1,2,*
1
College of Veterinary Medicine, South China Agricultural University, Guangzhou 510000, China
2
Key Laboratory of Comprehensive Prevention and Control for Severe Clinical Animal Diseases of Guangdong Province, Guangzhou 510000, China
These authors contributed equally to this study.
*
Author to whom correspondence should be addressed.
Academic Editor: Andrew Mehle
Received: 6 April 2016 / Revised: 24 May 2016 / Accepted: 26 May 2016 / Published: 31 May 2016
(This article belongs to the Section Animal Viruses)
View Full-Text   |   Download PDF [2624 KB, uploaded 1 June 2016]   |  

Abstract

An influenza virus polymerase reconstitution assay based on the human, dog, or chicken RNA polymerase I (PolI) promoter has been developed and widely used to study the polymerase activity of the influenza virus in corresponding cell types. Although it is an important member of the influenza virus family and has been known for sixty years, no studies have been performed to clone the horse PolI promoter or to study the polymerase activity of equine influenza virus (EIV) in horse cells. In our study, the horse RNA PolI promoter was cloned from fetal equine lung cells. Using the luciferase assay, it was found that a 500 bp horse RNA PolI promoter sequence was required for efficient transcription. Then, using the developed polymerase reconstitution assay based on the horse RNA PolI promoter, the polymerase activity of two EIV strains was compared, and equine myxovirus resistance A protein was identified as having the inhibiting EIV polymerase activity function in horse cells. Our study enriches our knowledge of the RNA PolI promoter of eukaryotic species and provides a useful tool for the study of influenza virus polymerase activity in horse cells. View Full-Text
Keywords: RNA polymerase I promoter; equine influenza virus; polymerase activity RNA polymerase I promoter; equine influenza virus; polymerase activity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Lu, G.; He, D.; Wang, Z.; Ou, S.; Yuan, R.; Li, S. Cloning the Horse RNA Polymerase I Promoter and Its Application to Studying Influenza Virus Polymerase Activity. Viruses 2016, 8, 119.

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