Next Article in Journal / Special Issue
Experimental Infection of Calves by Two Genetically-Distinct Strains of Rift Valley Fever Virus
Previous Article in Journal
Aerosol Transmission of Filoviruses
Previous Article in Special Issue
Early Bunyavirus-Host Cell Interactions
Article Menu

Export Article

Open AccessArticle
Viruses 2016, 8(5), 149; doi:10.3390/v8050149

N-Glycans on the Rift Valley Fever Virus Envelope Glycoproteins Gn and Gc Redundantly Support Viral Infection via DC-SIGN

1
Department of Pathology, The University of Texas Medical Branch, Galveston, TX 77555, USA
2
Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, TX 77555, USA
3
The Sealy Center for Vaccine Development, The University of Texas Medical Branch, Galveston, TX 77555, USA
4
The Center for Biodefense and Emerging Infectious Diseases, The University of Texas Medical Branch, Galveston, TX 77555, USA
5
Galveston National Laboratory, The University of Texas Medical Branch, Galveston, TX 77555, USA
Present address: Applied Research Associates, Alexandria, VA 22314-4576, USA
Present address: Novartis Institute for Biomedical Research, Cambridge, MA 02139, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Jane Tao and Pierre-Yves Lozach
Received: 8 March 2016 / Revised: 18 May 2016 / Accepted: 20 May 2016 / Published: 23 May 2016
(This article belongs to the Special Issue Recent Progress in Bunyavirus Research)
View Full-Text   |   Download PDF [1572 KB, uploaded 23 May 2016]   |  

Abstract

Rift Valley fever is a mosquito-transmitted, zoonotic disease that infects humans and ruminants. Dendritic cell specific intercellular adhesion molecule 3 (ICAM-3) grabbing non-integrin (DC-SIGN) acts as a receptor for members of the phlebovirus genus. The Rift Valley fever virus (RVFV) glycoproteins (Gn/Gc) encode five putative N-glycan sequons (asparagine (N)–any amino acid (X)–serine (S)/threonine (T)) at positions: N438 (Gn), and N794, N829, N1035, and N1077 (Gc). The N-glycosylation profile and significance in viral infection via DC-SIGN have not been elucidated. Gc N-glycosylation was first evaluated by using Gc asparagine (N) to glutamine (Q) mutants. Subsequently, we generated a series of recombinant RVFV MP-12 strain mutants, which encode N-to-Q mutations, and the infectivity of each mutant in Jurkat cells stably expressing DC-SIGN was evaluated. Results showed that Gc N794, N1035, and N1077 were N-glycosylated but N829 was not. Gc N1077 was heterogeneously N-glycosylated. RVFV Gc made two distinct N-glycoforms: “Gc-large” and “Gc-small”, and N1077 was responsible for “Gc-large” band. RVFV showed increased infection of cells expressing DC-SIGN compared to cells lacking DC-SIGN. Infection via DC-SIGN was increased in the presence of either Gn N438 or Gc N1077. Our study showed that N-glycans on the Gc and Gn surface glycoproteins redundantly support RVFV infection via DC-SIGN. View Full-Text
Keywords: Rift Valley fever virus; N-glycosylation; Gn; Gc; sequon; DC-SIGN; L-SIGN Rift Valley fever virus; N-glycosylation; Gn; Gc; sequon; DC-SIGN; L-SIGN
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Phoenix, I.; Nishiyama, S.; Lokugamage, N.; Hill, T.E.; Huante, M.B.; Slack, O.A.; Carpio, V.H.; Freiberg, A.N.; Ikegami, T. N-Glycans on the Rift Valley Fever Virus Envelope Glycoproteins Gn and Gc Redundantly Support Viral Infection via DC-SIGN. Viruses 2016, 8, 149.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top