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Viruses 2016, 8(12), 322; doi:10.3390/v8120322

Chloroquine, an Endocytosis Blocking Agent, Inhibits Zika Virus Infection in Different Cell Models

1
Department of Genetics, Institute of Biology, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, Brazil
2
Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, Brazil
3
D’Or Institute for Research and Education (IDOR), Rio de Janeiro 22281-100, Brazil
4
Department of Immunology, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, Brazil
5
Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, WI 53706, USA
6
Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI 53706, USA
7
Istituto Superiore di Sanità, Deptartment of Infectious Diseases, 299 Viale Regina Elena, 00161 Rome, Italy
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Michael R. Holbrook
Received: 16 October 2016 / Revised: 16 November 2016 / Accepted: 18 November 2016 / Published: 29 November 2016
(This article belongs to the Special Issue Advances in Flavivirus Research)
View Full-Text   |   Download PDF [2778 KB, uploaded 29 November 2016]   |  

Abstract

Zika virus (ZIKV) infection in utero might lead to microcephaly and other congenital defects. Since no specific therapy is available thus far, there is an urgent need for the discovery of agents capable of inhibiting its viral replication and deleterious effects. Chloroquine is widely used as an antimalarial drug, anti-inflammatory agent, and it also shows antiviral activity against several viruses. Here we show that chloroquine exhibits antiviral activity against ZIKV in Vero cells, human brain microvascular endothelial cells, human neural stem cells, and mouse neurospheres. We demonstrate that chloroquine reduces the number of ZIKV-infected cells in vitro, and inhibits virus production and cell death promoted by ZIKV infection without cytotoxic effects. In addition, chloroquine treatment partially reveres morphological changes induced by ZIKV infection in mouse neurospheres. View Full-Text
Keywords: Zika virus; chloroquine; antiviral; microcephaly; neural stem cell Zika virus; chloroquine; antiviral; microcephaly; neural stem cell
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Delvecchio, R.; Higa, L.M.; Pezzuto, P.; Valadão, A.L.; Garcez, P.P.; Monteiro, F.L.; Loiola, E.C.; Dias, A.A.; Silva, F.J.M.; Aliota, M.T.; Caine, E.A.; Osorio, J.E.; Bellio, M.; O’Connor, D.H.; Rehen, S.; de Aguiar, R.S.; Savarino, A.; Campanati, L.; Tanuri, A. Chloroquine, an Endocytosis Blocking Agent, Inhibits Zika Virus Infection in Different Cell Models. Viruses 2016, 8, 322.

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