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Viruses 2016, 8(10), 272; doi:10.3390/v8100272

A Viral Noncoding RNA Complements a Weakened Viral RNA Silencing Suppressor and Promotes Efficient Systemic Host Infection

1
Institut de Biologie Moléculaire des Plantes, Integrative Virology, CNRS UPR2367, Université de Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg, France
2
SESVanderHave, Industriepark soldatenplein, Z2nr15, Tienen B3300, Belgium
3
Dipartimento di Scienze Agrarie, Area Patologia Vegetale, Università di Bologna, Viale Fanin 40, 40127 Bologna, Italy
*
Author to whom correspondence should be addressed.
Received: 13 July 2016 / Accepted: 27 September 2016 / Published: 4 October 2016
(This article belongs to the Section Viruses of Plants, Fungi and Protozoa)
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Abstract

Systemic movement of beet necrotic yellow vein virus (BNYVV) in Beta macrocarpa depends on viral RNA3, whereas in Nicotiana benthamiana this RNA is dispensable. RNA3 contains a coremin motif of 20 nucleotides essential for the stabilization of noncoding RNA3 (ncRNA3) and for long‐distance movement in Beta species. Coremin mutants that are unable to accumulate ncRNA3 also do not achieve systemic movement in Beta species. A mutant virus carrying a mutation in the p14 viral suppressor of RNA silencing (VSR), unable to move long distances, can be complemented with the ncRNA3 in the lesion phenotype, viral RNA accumulation, and systemic spread. Analyses of the BNYVV VSR mechanism of action led to the identification of the RNA‐dependent RNA polymerase 6 (RDR6) pathway as a target of the virus VSR and the assignment of a VSR function to the ncRNA3. View Full-Text
Keywords: BNYVV; RSS; RNA silencing suppression; systemic movement; viral noncoding RNA BNYVV; RSS; RNA silencing suppression; systemic movement; viral noncoding RNA
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Flobinus, A.; Hleibieh, K.; Klein, E.; Ratti, C.; Bouzoubaa, S.; Gilmer, D. A Viral Noncoding RNA Complements a Weakened Viral RNA Silencing Suppressor and Promotes Efficient Systemic Host Infection. Viruses 2016, 8, 272.

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