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Viruses 2016, 8(1), 14; doi:10.3390/v8010014

Metaviromics of Namib Desert Salt Pans: A Novel Lineage of Haloarchaeal Salterproviruses and a Rich Source of ssDNA Viruses

1
Centre for Microbial Ecology and Genomics, Genomics Research Institute, University of Pretoria, Natural Sciences II, Lynnwood Road, 0002 Pretoria, South Africa
2
Institute for Microbial Biotechnology and Metagenomics, University of the Western Cape, 7535 Bellville, Cape Town, South Africa
*
Author to whom correspondence should be addressed.
Academic Editor: Rob Lavigne
Received: 22 October 2015 / Revised: 26 November 2015 / Accepted: 14 December 2015 / Published: 8 January 2016
(This article belongs to the Section Bacterial Viruses)
View Full-Text   |   Download PDF [2235 KB, uploaded 8 January 2016]   |  

Abstract

Viral communities of two different salt pans located in the Namib Desert, Hosabes and Eisfeld, were investigated using a combination of multiple displacement amplification of metaviromic DNA and deep sequencing, and provided comprehensive sequence data on both ssDNA and dsDNA viral community structures. Read and contig annotations through online pipelines showed that the salt pans harbored largely unknown viral communities. Through network analysis, we were able to assign a large portion of the unknown reads to a diverse group of ssDNA viruses. Contigs belonging to the subfamily Gokushovirinae were common in both environmental datasets. Analysis of haloarchaeal virus contigs revealed the presence of three contigs distantly related with His1, indicating a possible new lineage of salterproviruses in the Hosabes playa. Based on viral richness and read mapping analyses, the salt pan metaviromes were novel and most closely related to each other while showing a low degree of overlap with other environmental viromes. View Full-Text
Keywords: Namib Desert playa; metaviromics; environmental viromics; hypersaline; haloarchaeal viruses; ssDNA viruses; Gokushovirinae; Microviridae Namib Desert playa; metaviromics; environmental viromics; hypersaline; haloarchaeal viruses; ssDNA viruses; Gokushovirinae; Microviridae
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Adriaenssens, E.M.; van Zyl, L.J.; Cowan, D.A.; Trindade, M.I. Metaviromics of Namib Desert Salt Pans: A Novel Lineage of Haloarchaeal Salterproviruses and a Rich Source of ssDNA Viruses. Viruses 2016, 8, 14.

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