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Viruses 2016, 8(1), 10; doi:10.3390/v8010010

Direct Identification of Enteroviruses in Cerebrospinal Fluid of Patients with Suspected Meningitis by Nested PCR Amplification

1
Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119899, Russia
2
Chumakov Institute of Poliomyelitis and Viral Encephalitides, Moscow 142782, Russia
3
Hygienic and Epidemiological Center in the Omsk Region, Omsk 644116, Russia
*
Author to whom correspondence should be addressed.
Academic Editor: George Belov
Received: 23 October 2015 / Revised: 21 December 2015 / Accepted: 24 December 2015 / Published: 6 January 2016
(This article belongs to the Special Issue Recent Progress in Enterovirus Research)
View Full-Text   |   Download PDF [682 KB, uploaded 6 January 2016]   |  

Abstract

Enteroviruses, the most common human viral pathogens worldwide, have been associated with serous meningitis, encephalitis, syndrome of acute flaccid paralysis, myocarditis and the onset of diabetes type 1. In the future, the rapid identification of the etiological agent would allow to adjust the therapy promptly and thereby improve the course of the disease and prognosis. We developed RT-nested PCR amplification of the genomic region coding viral structural protein VP1 for direct identification of enteroviruses in clinical specimens and compared it with the existing analogs. One-hundred-fifty-nine cerebrospinal fluids (CSF) from patients with suspected meningitis were studied. The amplification of VP1 genomic region using the new method was achieved for 86 (54.1%) patients compared with 75 (47.2%), 53 (33.3%) and 31 (19.5%) achieved with previously published methods. We identified 11 serotypes of the Enterovirus species B in 2012, including relatively rare echovirus 14 (E-14), E-15 and E-32, and eight serotypes of species B and 5 enteroviruses A71 (EV-A71) in 2013. The developed method can be useful for direct identification of enteroviruses in clinical material with the low virus loads such as CSF. View Full-Text
Keywords: Enterovirus; nested PCR; cerebrospinal fluids (CSF); direct genotyping; detection; identification; sequencing Enterovirus; nested PCR; cerebrospinal fluids (CSF); direct genotyping; detection; identification; sequencing
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Krasota, A.; Loginovskih, N.; Ivanova, O.; Lipskaya, G. Direct Identification of Enteroviruses in Cerebrospinal Fluid of Patients with Suspected Meningitis by Nested PCR Amplification. Viruses 2016, 8, 10.

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