Next Article in Journal
Recombinant Immunomodulating Lentogenic or Mesogenic Oncolytic Newcastle Disease Virus for Treatment of Pancreatic Adenocarcinoma
Next Article in Special Issue
RNase P Ribozymes Inhibit the Replication of Human Cytomegalovirus by Targeting Essential Viral Capsid Proteins
Previous Article in Journal
The Chikungunya Virus Capsid Protein Contains Linear B Cell Epitopes in the N- and C-Terminal Regions that are Dependent on an Intact C-Terminus for Antibody Recognition
Previous Article in Special Issue
Alphaviruses in Gene Therapy
Article Menu

Export Article

Open AccessArticle
Viruses 2015, 7(6), 2965-2979; doi:10.3390/v7062755

Lentiviral Vector Mediated Claudin1 Silencing Inhibits Epithelial to Mesenchymal Transition in Breast Cancer Cells

1,2,3,†
,
1,2,3,†
,
2,3
,
2,3
,
2,3
,
2,3
and
2,3,*
1
Department of Medicine, Harbin Medical University, Harbin 150086, China
2
Department of Pathology and Laboratory Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
3
Center for Cancer Research, University of Tennessee Health Science Center, 19 S. Manassas St., Rm. 266, Memphis, TN 38163, USA
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Kenneth Lundstrom
Received: 17 February 2015 / Revised: 31 March 2015 / Accepted: 4 June 2015 / Published: 10 June 2015
(This article belongs to the Special Issue Gene Therapy with Emphasis on RNA Interference)
View Full-Text   |   Download PDF [1051 KB, uploaded 10 June 2015]   |  

Abstract

Breast cancer has a high incidence and mortality rate worldwide. Several viral vectors including lentiviral, adenoviral and adeno-associated viral vectors have been used in gene therapy for various forms of human cancer, and have shown promising effects in controlling tumor development. Claudin1 (CLDN1) is a member of the tetraspan transmembrane protein family that plays a major role in tight junctions and is associated with tumor metastasis. However, the role of CLDN1 in breast cancer is largely unexplored. In this study, we tested the therapeutic potential of silencing CLDN1 expression in two breast cancer (MDA-MB-231 and MCF7) cell lines using lentiviral vector mediated RNA interference. We found that a CLDN1 short hairpin (shRNA) construct efficiently silenced CLDN1 expression in both breast cancer cell lines, and CLDN1 knockdown resulted in reduced cell proliferation, survival, migration and invasion. Furthermore, silencing CLDN1 inhibited epithelial to mesenchymal transition (EMT) by upregulating the epithelial cell marker, E-cadherin, and downregulating mesenchymal markers, smooth muscle cell alpha-actin (SMA) and Snai2. Our data demonstrated that lentiviral vector mediated CLDN1 RNA interference has great potential in breast cancer gene therapy by inhibiting EMT and controlling tumor cell growth. View Full-Text
Keywords: CLDN1; epithelial to mesenchymal transition; breast cancer CLDN1; epithelial to mesenchymal transition; breast cancer
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Zhao, X.; Zou, Y.; Gu, Q.; Zhao, G.; Gray, H.; Pfeffer, L.M.; Yue, J. Lentiviral Vector Mediated Claudin1 Silencing Inhibits Epithelial to Mesenchymal Transition in Breast Cancer Cells. Viruses 2015, 7, 2965-2979.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top