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Viruses 2015, 7(4), 1832-1852; doi:10.3390/v7041832

In Ovo Delivery of CpG DNA Reduces Avian Infectious Laryngotracheitis Virus Induced Mortality and Morbidity

1
Faculty of Veterinary Medicine, University of Calgary, Health Research Innovation Center 2C64, 3330 Hospital Drive NW, Calgary, AB T2N 4N1, Canada
2
Department of Pathobiology, University of Guelph, Guelph, ON N1G 2W1, Canada
Current address: Faculty of Medicine, University of Jaffna, Jaffna 40000, Sri Lanka.
*
Author to whom correspondence should be addressed.
Academic Editor: Curt Hagedorn
Received: 20 December 2014 / Revised: 8 March 2015 / Accepted: 26 March 2015 / Published: 8 April 2015
(This article belongs to the Section Antivirals & Vaccines)
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Abstract

Endosomal toll-like receptor-21 and -9 sense CpG DNA activating production of pro-inflammatory mediators with antimicrobial effects. Here, we investigated the induction of antiviral response of in ovo delivered CpG DNA against infectious laryngotracheitis virus (ILTV) infection. We found that in ovo delivered CpG DNA significantly reduces ILTV infection pre-hatch correlating with the expression of IL-1β and increase of macrophages in lungs. As assessed in vitro, CpG DNA stimulated avian macrophages could be a potential source of IL-1β and other pro-inflammatory mediators. Since we also found that in ovo CpG DNA delivery maintains increased macrophages in the lungs post-hatch, we infected the chickens on the day of hatch with ILTV. We found that in ovo delivered CpG DNA significantly reduces mortality and morbidity resulting from ILTV infection encountered post-hatch. Thus, CpG DNA can be a candidate innate immune stimulant worthy of further investigation for the control of ILTV infection in chickens. View Full-Text
Keywords: In ovo; toll-like receptor 21; CpG-motif containing synthetic DNA; infectious laryngotracheitis virus; lung; chicken In ovo; toll-like receptor 21; CpG-motif containing synthetic DNA; infectious laryngotracheitis virus; lung; chicken
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Thapa, S.; Cader, M.S.A.; Murugananthan, K.; Nagy, E.; Sharif, S.; Czub, M.; Abdul-Careem, M.F. In Ovo Delivery of CpG DNA Reduces Avian Infectious Laryngotracheitis Virus Induced Mortality and Morbidity. Viruses 2015, 7, 1832-1852.

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