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Viruses 2014, 6(7), 2723-2734; doi:10.3390/v6072723

Increased Plasma Cell-Free DNA Level during HTNV Infection: Correlation with Disease Severity and Virus Load

1
Department of Immunology, The Fourth Military Medical University, 169 Changle West Rd, Xi'an 710032, China
2
Department of Blood Transfusion, Xijing Hospital, The Fourth Military Medical University, 15 Changle West Rd, Xi'an 710032, China
3
Department of Clinical Laboratory, Xijing Hospital, The Fourth Military Medical University, 15 Changle West Rd, Xi'an 710032, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 29 November 2013 / Revised: 13 June 2014 / Accepted: 29 June 2014 / Published: 15 July 2014
(This article belongs to the Special Issue Hantaviruses)
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Abstract

Cell-free DNA (cf-DNA) in blood represents a promising DNA damage response triggered by virus infection or trauma, tumor, etc. Hantavirus primarily causes two diseases: haemorrhagic fever with renal syndrome (HFRS) and Hantavirus cardiopulmonary syndrome (HCPS), depending on different Hantavirus species. The aim of this study was to evaluate plasma cf-DNA levels in acute phase of HFRS, and to correlate plasma cf-DNA with disease severity and plasma Hanttan virus (HTNV) load. We observed the appearance of cf-DNA in 166 plasma samples from 76 HFRS patients: the plasma cf-DNA levels peaked at the hypotensive stage of HFRS, and then decreased gradually. Until the diuretic stage, there was no significant difference in plasma cf-DNA level between patients and the healthy control. Exclusively in the febrile/hypotensive stage, the plasma cf-DNA levels of severe/critical patients were higher than those of the mild/moderate group. Moreover, the plasma cf-DNA value in the early stage of HFRS was correlated with HTNV load and disease severity. In most of the patients, plasma cf-DNA displayed a low-molecular weight appearance, corresponding to the size of apoptotic DNA. In conclusion, the plasma cf-DNA levels were dynamically elevated during HFRS, and correlated with disease severity, which suggests that plasma cf-DNA may be a potential biomarker for the pathogenesis and prognosis of HFRS. View Full-Text
Keywords: cf-DNA; HFRS; Hantaan virus; disease severity cf-DNA; HFRS; Hantaan virus; disease severity
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Yi, J.; Zhang, Y.; Zhang, Y.; Ma, Y.; Zhang, C.; Li, Q.; Liu, B.; Liu, Z.; Liu, J.; Zhang, X.; Zhuang, R.; Jin, B. Increased Plasma Cell-Free DNA Level during HTNV Infection: Correlation with Disease Severity and Virus Load. Viruses 2014, 6, 2723-2734.

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