Genetic Diversity in HIV-1 Subtype C LTR from Brazil and Mozambique Generates New Transcription Factor-Binding Sites
Abstract
:1. Introduction
2. Results and Discussion
Sample no | Sample Code | Subtype | CD4 (cell/mm3) | Viral Load (copies/mL) | Presence of MFNLP | ||||
---|---|---|---|---|---|---|---|---|---|
LTR | PR | RT | NF-κB F | NF-κB like | RBE-III | ||||
1 | M-7 | C | - | C | 314 | 61.252 | − | + | + |
2 | M-9 | C | - | C | 731 | 3.312 | − | − | − |
3 | M-22 | C | - | C | 303 | 12.745 | − | + | + |
4 | M-24 | C | - | C | 339 | 27.756 | + | − | − |
5 | M-47 | C | - | C | 235 | 11.246 | + | − | − |
6 | M-54 | C | - | C | 231 | 92.079 | − | − | − |
7 | M-66 | C | - | C | 574 | 2.982 | + | − | − |
8 | M-72 | C | - | C | 561 | 490.032 | − | − | − |
9 | M-81 | C | - | C | 554 | 6.796 | − | − | − |
10 | PR-G1 362 | C | C | C | 765 | 156 | + | − | − |
11 | PR-G1 418 | C | C | C | 815 | 105.948 | − | − | + |
12 | PR-G1 422 | C | C | C | 523 | 36.696 | + | − | − |
13 | PR-G1 441 | C | C | C | 523 | 17.279 | − | − | − |
14 | PR-G1 442 | C | C | C | 637 | 3.772 | + | − | − |
15 | PR-G3 60 | C | C | C | 148 | - | − | − | + |
16 | PR-G3 77 | C | C | C | - | - | − | − | − |
17 | PR-G3 85 | C | C | C | 89 | 10 | − | − | − |
18 | PR-G3 111 | B | C | C | 218 | 29.728 | − | − | − |
19 | PR-G3 254 | D | C | C | - | - | − | − | − |
20 | PR-G3 319 | C | C | C | 80 | 340.000 | − | − | − |
21 | PR-G3 370 | C | C | C | 28 | - | − | − | + |
22 | RS-G1 27 | C | C | C | - | - | − | − | − |
23 | RS-G1 30 | C | C | C | - | - | − | − | − |
24 | RS-G1 37 | C | C | C | 15,406 | 842 | − | − | − |
25 | RS-G1 39 | C | C | C | 438 | 8.234 | − | − | − |
26 | RS-G1 45 | C | C | C | 655 | 564 | − | − | + |
27 | RS-G1 109 | C | C | C | 721 | 656 | − | − | + |
28 | RS-G1 112 | C | C | C | 1356 | 140 | − | − | + |
29 | RS-G1 136 | C | C | C | 877 | 9.284 | − | − | + |
30 | RS-G3 294 | C | C | C | 190 | 249 | − | − | − |
31 | RS-G3 307 | C | C | C | - | - | − | − | − |
3. Experimental Section
3.1. Samples
3.2. PCR Amplification
3.3. Sequencing
3.4. Sequence Analysis
4. Conclusions
Supplementary Files
Acknowledgments
Author Contributions
Conflicts of Interest
References and Notes
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Boullosa, J.; Bachu, M.; Bila, D.; Ranga, U.; Süffert, T.; Sasazawa, T.; Tanuri, A. Genetic Diversity in HIV-1 Subtype C LTR from Brazil and Mozambique Generates New Transcription Factor-Binding Sites. Viruses 2014, 6, 2495-2504. https://doi.org/10.3390/v6062495
Boullosa J, Bachu M, Bila D, Ranga U, Süffert T, Sasazawa T, Tanuri A. Genetic Diversity in HIV-1 Subtype C LTR from Brazil and Mozambique Generates New Transcription Factor-Binding Sites. Viruses. 2014; 6(6):2495-2504. https://doi.org/10.3390/v6062495
Chicago/Turabian StyleBoullosa, José, Mahesh Bachu, Dulce Bila, Udaykumar Ranga, Theodoro Süffert, Tomoko Sasazawa, and Amilcar Tanuri. 2014. "Genetic Diversity in HIV-1 Subtype C LTR from Brazil and Mozambique Generates New Transcription Factor-Binding Sites" Viruses 6, no. 6: 2495-2504. https://doi.org/10.3390/v6062495