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Viruses 2014, 6(3), 1424-1441; doi:10.3390/v6031424
Article

IFN-Dependent and -Independent Reduction in West Nile Virus Infectivity in Human Dermal Fibroblasts

1,2
 and 1,2,*
Received: 22 January 2014; in revised form: 5 March 2014 / Accepted: 7 March 2014 / Published: 24 March 2014
(This article belongs to the Section Animal Viruses)
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Abstract: Although dermal fibroblasts are one of the first cell types exposed to West Nile virus (WNV) during a blood meal by an infected mosquito, little is known about WNV replication within this cell type. Here, we demonstrate that neuroinvasive, WNV-New York (WNV-NY), and nonneuroinvasive, WNV-Australia (WNV-AUS60) strains are able to infect and replicate in primary human dermal fibroblasts (HDFs). However, WNV-AUS60 replication and spread within HDFs was reduced compared to that of WNV-NY due to an interferon (IFN)-independent reduction in viral infectivity early in infection. Additionally, replication of both strains was constrained late in infection by an IFN-β-dependent reduction in particle infectivity. Overall, our data indicates that human dermal fibroblasts are capable of supporting WNV replication; however, the low infectivity of particles produced from HDFs late in infection suggests that this cell type likely plays a limited role as a viral reservoir in vivo.
Keywords: West Nile virus; interferon; human dermal fibroblast; antiviral response; intracellular innate immunity West Nile virus; interferon; human dermal fibroblast; antiviral response; intracellular innate immunity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Hoover, L.I.; Fredericksen, B.L. IFN-Dependent and -Independent Reduction in West Nile Virus Infectivity in Human Dermal Fibroblasts. Viruses 2014, 6, 1424-1441.

AMA Style

Hoover LI, Fredericksen BL. IFN-Dependent and -Independent Reduction in West Nile Virus Infectivity in Human Dermal Fibroblasts. Viruses. 2014; 6(3):1424-1441.

Chicago/Turabian Style

Hoover, Lisa I.; Fredericksen, Brenda L. 2014. "IFN-Dependent and -Independent Reduction in West Nile Virus Infectivity in Human Dermal Fibroblasts." Viruses 6, no. 3: 1424-1441.


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