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Viruses 2014, 6(3), 1004-1014; doi:10.3390/v6031004

Isolation and Identification of Feline Herpesvirus Type 1 from a South China Tiger in China

1
College of Wildlife Resource, Northeast Forestry University, Haerbin, 150040, China
2
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, 730070, China
3
Wildlife Rescue and Breeding Centre of Jilin Province, Changchun, 130122, China
4
Shenzhen Wildlife Zoo, Shenzhen, 518055, China
5
Military Veterinary Research Institute of Academy of Military Medical Sciences, Changchun, 130122, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 10 November 2013 / Revised: 26 January 2014 / Accepted: 20 February 2014 / Published: 28 February 2014
(This article belongs to the Section Animal Viruses)
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Abstract

In 2012, an FHV-1-like virus was isolated from a tiger that presented with clinical signs of sialorrhea, sneezing and purulent rhinorrhea. Isolation was performed with the FK81 cell line, and the virus was identified by PCR, transmission electron microscopy (TEM), and the phylogenetic analysis of the partial thymidine kinase (TK) and glycoprotein B (gB) genes. A total of 253 bp of the TK gene and 566 bp of the gB gene were amplified from the trachea of the tiger by PCR/RT-PCR method. Phylogenetic analysis showed that the isolate belonged to the same cluster with other FHV-1 strains obtained from GenBank. Herpes-like viruses with an envelope and diameters of approximately 200 nm were observed in the culture supernatants of FK81 cells inoculated with samples from the tiger. The FHV-1 infection was confirmed by an animal challenge experiment in a cat model. Our finding extends the host range of FHV-1 and has implications for FHV-1 infection and South China tiger conservation. View Full-Text
Keywords: feline herpesvirus type 1; virus isolation; animal challenge experiment; South China tiger feline herpesvirus type 1; virus isolation; animal challenge experiment; South China tiger
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Sun, H.; Li, Y.; Jiao, W.; Liu, C.; Liu, X.; Wang, H.; Hua, F.; Dong, J.; Fan, S.; Yu, Z.; Gao, Y.; Xia, X. Isolation and Identification of Feline Herpesvirus Type 1 from a South China Tiger in China. Viruses 2014, 6, 1004-1014.

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