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Viruses 2014, 6(11), 4195-4211; doi:10.3390/v6114195

Cytoplasmic Dynein Promotes HIV-1 Uncoating

Laboratory of Retrovirology, Department of Medical Biology and BioMed Research Center, Université du Québec à Trois-Rivières, 3351 Boulevard des Forges, Trois-Rivières, QC G9A 5H7, Canada
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Received: 2 October 2014 / Revised: 24 October 2014 / Accepted: 27 October 2014 / Published: 4 November 2014
(This article belongs to the Section Animal Viruses)
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Abstract

Retroviral capsid (CA) cores undergo uncoating during their retrograde transport (toward the nucleus), and/or after reaching the nuclear membrane. However, whether HIV-1 CA core uncoating is dependent upon its transport is not understood. There is some evidence that HIV-1 cores retrograde transport involves cytoplasmic dynein complexes translocating on microtubules. Here we investigate the role of dynein-dependent transport in HIV-1 uncoating. To interfere with dynein function, we depleted dynein heavy chain (DHC) using RNA interference, and we over-expressed p50/dynamitin. In immunofluorescence microscopy experiments, DHC depletion caused an accumulation of CA foci in HIV-1 infected cells. Using a biochemical assay to monitor HIV-1 CA core disassembly in infected cells, we observed an increase in amounts of intact (pelletable) CA cores upon DHC depletion or p50 over-expression. Results from these two complementary assays suggest that inhibiting dynein-mediated transport interferes with HIV-1 uncoating in infected cells, indicating the existence of a functional link between HIV-1 transport and uncoating. View Full-Text
Keywords: HIV-1; uncoating; capsid; microtubules; dynein HIV-1; uncoating; capsid; microtubules; dynein
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Pawlica, P.; Berthoux, L. Cytoplasmic Dynein Promotes HIV-1 Uncoating. Viruses 2014, 6, 4195-4211.

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