Next Article in Journal
Next Article in Special Issue
Previous Article in Journal
Previous Article in Special Issue
Viruses 2013, 5(2), 758-776; doi:10.3390/v5020758
Article

Localization, Concentration, and Transmission Efficiency of Banana bunchy top virus in Four Asexual Lineages of Pentalonia aphids

1,2
, 1,3
 and 1,*
Received: 21 January 2013; in revised form: 14 February 2013 / Accepted: 14 February 2013 / Published: 22 February 2013
(This article belongs to the Special Issue Plant Viruses)
View Full-Text   |   Download PDF [797 KB, uploaded 25 February 2013]
Abstract: Banana bunchy top virus (BBTV) is the most destructive pathogenic virus of banana plants worldwide. The virus is transmitted in a circulative non-propagative manner by the banana aphid, Pentalonia nigronervosa Coquerel. In this work, we examined the localization, accumulation, and transmission efficiency of BBTV in four laboratory-established lineages of Pentalonia aphids derived from four different host plants: taro (Colocasia esculenta), heliconia (Heliconia spp.), red ginger (Alpinia purpurata), and banana (Musa sp.). Mitochondrial sequencing identified three and one lineages as Pentalonia caladii van der Goot, a recently proposed species, and P. nigronervosa, respectively. Microsatellite analysis separated the aphid lineages into four distinct genotypes. The transmission of BBTV was tested using leaf disk and whole-plant assays, both of which showed that all four lineages are competent vectors of BBTV, although the P. caladii from heliconia transmitted BBTV to the leaf disks at a significantly lower rate than did P. nigronervosa. The concentration of BBTV in dissected guts, haemolymph, and salivary glands was quantified by real-time PCR. The BBTV titer reached similar concentrations in the guts, haemolymph, and salivary glands of aphids from all four lineages tested. Furthermore, immunofluorescence assays showed that BBTV antigens localized to the anterior midguts and the principal salivary glands, demonstrating a similar pattern of translocations across the four lineages. The results reported in this study showed for the first time that P. caladii is a competent vector of BBTV.
Keywords: Pentalonia nigronervosa; Pentalonia caladii; immunofluorescence localization; real time PCR Pentalonia nigronervosa; Pentalonia caladii; immunofluorescence localization; real time PCR
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Watanabe, S.; Greenwell, A.M.; Bressan, A. Localization, Concentration, and Transmission Efficiency of Banana bunchy top virus in Four Asexual Lineages of Pentalonia aphids. Viruses 2013, 5, 758-776.

AMA Style

Watanabe S, Greenwell AM, Bressan A. Localization, Concentration, and Transmission Efficiency of Banana bunchy top virus in Four Asexual Lineages of Pentalonia aphids. Viruses. 2013; 5(2):758-776.

Chicago/Turabian Style

Watanabe, Shizu; Greenwell, April M.; Bressan, Alberto. 2013. "Localization, Concentration, and Transmission Efficiency of Banana bunchy top virus in Four Asexual Lineages of Pentalonia aphids." Viruses 5, no. 2: 758-776.



Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert