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Localization, Concentration, and Transmission Efficiency of Banana bunchy top virus in Four Asexual Lineages of Pentalonia aphids
Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Honolulu, HI 96822, USA
Department of Molecular Bioscience and Bioengineering, University of Hawaii, Honolulu, HI 96822, USA
NSF-Center for Integrated Pest Management, North Carolina State University, USDA APHIS PPQ office, Honolulu, HI 96850, USA
* Author to whom correspondence should be addressed.
Received: 21 January 2013; in revised form: 14 February 2013 / Accepted: 14 February 2013 / Published: 22 February 2013
Abstract: Banana bunchy top virus (BBTV) is the most destructive pathogenic virus of banana plants worldwide. The virus is transmitted in a circulative non-propagative manner by the banana aphid, Pentalonia nigronervosa Coquerel. In this work, we examined the localization, accumulation, and transmission efficiency of BBTV in four laboratory-established lineages of Pentalonia aphids derived from four different host plants: taro (Colocasia esculenta), heliconia (Heliconia spp.), red ginger (Alpinia purpurata), and banana (Musa sp.). Mitochondrial sequencing identified three and one lineages as Pentalonia caladii van der Goot, a recently proposed species, and P. nigronervosa, respectively. Microsatellite analysis separated the aphid lineages into four distinct genotypes. The transmission of BBTV was tested using leaf disk and whole-plant assays, both of which showed that all four lineages are competent vectors of BBTV, although the P. caladii from heliconia transmitted BBTV to the leaf disks at a significantly lower rate than did P. nigronervosa. The concentration of BBTV in dissected guts, haemolymph, and salivary glands was quantified by real-time PCR. The BBTV titer reached similar concentrations in the guts, haemolymph, and salivary glands of aphids from all four lineages tested. Furthermore, immunofluorescence assays showed that BBTV antigens localized to the anterior midguts and the principal salivary glands, demonstrating a similar pattern of translocations across the four lineages. The results reported in this study showed for the first time that P. caladii is a competent vector of BBTV.
Keywords: Pentalonia nigronervosa; Pentalonia caladii; immunofluorescence localization; real time PCR
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Watanabe, S.; Greenwell, A.M.; Bressan, A. Localization, Concentration, and Transmission Efficiency of Banana bunchy top virus in Four Asexual Lineages of Pentalonia aphids. Viruses 2013, 5, 758-776.
Watanabe S, Greenwell AM, Bressan A. Localization, Concentration, and Transmission Efficiency of Banana bunchy top virus in Four Asexual Lineages of Pentalonia aphids. Viruses. 2013; 5(2):758-776.
Watanabe, Shizu; Greenwell, April M.; Bressan, Alberto. 2013. "Localization, Concentration, and Transmission Efficiency of Banana bunchy top virus in Four Asexual Lineages of Pentalonia aphids." Viruses 5, no. 2: 758-776.