Viruses 2013, 5(11), 2840-2855; doi:10.3390/v5112840
Article

HPV Genotyping 9G Membrane Test

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Received: 21 October 2013; in revised form: 6 November 2013 / Accepted: 19 November 2013 / Published: 22 November 2013
(This article belongs to the Section Animal Viruses)
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Abstract: The results of the genital human papillomavirus (HPV) detection in 439 cervical samples by cervical cytology were compared with sequencing analysis and a newly developed HPV genotyping 9G membrane test. The excellent sensitivity and specificity of the HPV genotyping 9G membrane test was assured by a signal to noise ratio of more than 300 and a target hybridization to non-target hybridization ratio of 300 ~ 400 at 25 °C. The final results can be obtained in 29 min by simple loading of the hybridization and washing solutions and scanning the membranes without any drying steps or special handling. The 100% identical results of the HPV genotyping 9G membrane test with sequencing results in 439 clinical samples demonstrate significant clinical application for this test. HPV genotyping 9G membrane tests can identify and discriminate five HR-HPV genotypes which are prevalent in almost 87% of cervical cancer cases. Its simple handling makes the HPV genotyping 9G membrane test a very convenient platform for accurate HPV genotyping.
Keywords: HPV; cervical neoplasia; genotyping; 9G membrane; cervical cancer
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Sayyed, D.R.; Song, K.-S.; Nimse, S.B.; An, H.; Kim, J.; Kim, T. HPV Genotyping 9G Membrane Test. Viruses 2013, 5, 2840-2855.

AMA Style

Sayyed DR, Song K-S, Nimse SB, An H, Kim J, Kim T. HPV Genotyping 9G Membrane Test. Viruses. 2013; 5(11):2840-2855.

Chicago/Turabian Style

Sayyed, Danishmalik R.; Song, Keum-Soo; Nimse, Satish B.; An, Heejung; Kim, Junghoon; Kim, Taisun. 2013. "HPV Genotyping 9G Membrane Test." Viruses 5, no. 11: 2840-2855.


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