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Authentication of the R06E Fruit Bat Cell Line
ProBioGen AG, Goethestr. 54, Berlin 13086, Germany
Rocky Mountain Laboratories, NIAID, NIH, 903 S 4th Street, Hamilton, MT 59840, USA
* Author to whom correspondence should be addressed.
Received: 2 May 2012; in revised form: 18 May 2012 / Accepted: 21 May 2012 / Published: 23 May 2012
Abstract: Fruit bats and insectivorous bats are believed to provide a natural reservoir for a wide variety of infectious diseases. Several lines of evidence, including the successful isolation of infectious viruses, indicate that Marburg virus and Ravn virus have found a major reservoir in colonies of the Egyptian rousette (Rousettus aegyptiacus). To facilitate molecular studies on virus-reservoir host interactions and isolation of viruses from environmental samples, we established cell lines from primary cells of this animal. The cell lines were given to several laboratories until we realized that a contamination with Vero cells in one of the cultures had occurred. Here we describe a general diagnostic procedure for identification of cross-species contamination with the focus on Vero and Rousettus cell lines, and summarize newly discovered properties of the cell lines that may pertain to pathogen discovery.
Keywords: fruit bat cell line; R06E; R05T; Rousettus aegyptiacus
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MDPI and ACS Style
Jordan, I.; Munster, V.J.; Sandig, V. Authentication of the R06E Fruit Bat Cell Line. Viruses 2012, 4, 889-900.
Jordan I, Munster VJ, Sandig V. Authentication of the R06E Fruit Bat Cell Line. Viruses. 2012; 4(5):889-900.
Jordan, Ingo; Munster, Vincent J.; Sandig, Volker. 2012. "Authentication of the R06E Fruit Bat Cell Line." Viruses 4, no. 5: 889-900.