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Viruses 2012, 4(2), 211-235; doi:10.3390/v4020211

Back to BAC: The Use of Infectious Clone Technologies for Viral Mutagenesis

1
School of Veterinary Science, The University of Queensland, Gatton, QLD 4343, Australia
2
Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD 4072, Australia
3
Queensland Agricultural Biotechnology Facility, Ritchie Laboratories, St Lucia, QLD 4067, Australia
*
Author to whom correspondence should be addressed.
Received: 20 December 2011 / Revised: 26 January 2012 / Accepted: 30 January 2012 / Published: 3 February 2012
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Abstract

Bacterial artificial chromosome (BAC) vectors were first developed to facilitate the propagation and manipulation of large DNA fragments in molecular biology studies for uses such as genome sequencing projects and genetic disease models. To facilitate these studies, methodologies have been developed to introduce specific mutations that can be directly applied to the mutagenesis of infectious clones (icBAC) using BAC technologies. This has resulted in rapid identification of gene function and expression at unprecedented rates. Here we review the major developments in BAC mutagenesis in vitro. This review summarises the technologies used to construct and introduce mutations into herpesvirus icBAC. It also explores developing technologies likely to provide the next leap in understanding these important viruses.
Keywords: chromosomes; artificial; bacterial; recombination; genetic; mutagenesis; cloning; molecular methods; transposition; DNA viruses; infectious clone chromosomes; artificial; bacterial; recombination; genetic; mutagenesis; cloning; molecular methods; transposition; DNA viruses; infectious clone
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Hall, R.N.; Meers, J.; Fowler, E.; Mahony, T. Back to BAC: The Use of Infectious Clone Technologies for Viral Mutagenesis. Viruses 2012, 4, 211-235.

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