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Viruses 2012, 4(2), 211-235; doi:10.3390/v4020211
Review

Back to BAC: The Use of Infectious Clone Technologies for Viral Mutagenesis

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Received: 20 December 2011 / Revised: 26 January 2012 / Accepted: 30 January 2012 / Published: 3 February 2012
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Abstract

Bacterial artificial chromosome (BAC) vectors were first developed to facilitate the propagation and manipulation of large DNA fragments in molecular biology studies for uses such as genome sequencing projects and genetic disease models. To facilitate these studies, methodologies have been developed to introduce specific mutations that can be directly applied to the mutagenesis of infectious clones (icBAC) using BAC technologies. This has resulted in rapid identification of gene function and expression at unprecedented rates. Here we review the major developments in BAC mutagenesis in vitro. This review summarises the technologies used to construct and introduce mutations into herpesvirus icBAC. It also explores developing technologies likely to provide the next leap in understanding these important viruses.
Keywords: chromosomes; artificial; bacterial; recombination; genetic; mutagenesis; cloning; molecular methods; transposition; DNA viruses; infectious clone chromosomes; artificial; bacterial; recombination; genetic; mutagenesis; cloning; molecular methods; transposition; DNA viruses; infectious clone
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Hall, R.N.; Meers, J.; Fowler, E.; Mahony, T. Back to BAC: The Use of Infectious Clone Technologies for Viral Mutagenesis. Viruses 2012, 4, 211-235.

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