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Viruses 2012, 4(12), 3804-3811; doi:10.3390/v4123804
Article

First Report of Cowpea Mild Mottle Carlavirus on Yardlong Bean (Vigna unguiculata subsp. sesquipedalis) in Venezuela

1
, 2,†, 1
, 2
, 2
 and 2,*
Received: 4 November 2012; in revised form: 6 December 2012 / Accepted: 11 December 2012 / Published: 14 December 2012
(This article belongs to the Section Viruses of Plants, Fungi and Protoza)
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Abstract: Yardlong bean (Vigna unguiculata subsp. sesquipedalis) plants with virus-like systemic mottling and leaf distortion were observed in both experimental and commercial fields in Aragua State, Venezuela. Symptomatic leaves were shown to contain carlavirus-like particles. RT-PCR analysis with carlavirus-specific primers was positive in all tested samples. Nucleotide sequences of the obtained amplicons showed 84%–74% similarity to corresponding sequences of Cowpea mild mottle virus (CPMMV) isolates deposited in the GenBank database. This is the first report of CPMMV in Venezuela and is thought to be the first report of CPMMV infecting yardlong bean.
Keywords: Vigna unguiculata; sesquipedalis; yardlong bean; Cowpea mild mottle carlavirus; CPMMV; diagnosis; RT-PCR; Venezuela Vigna unguiculata; sesquipedalis; yardlong bean; Cowpea mild mottle carlavirus; CPMMV; diagnosis; RT-PCR; Venezuela
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Brito, M.; Fernández-Rodríguez, T.; Garrido, M.J.; Mejías, A.; Romano, M.; Marys, E. First Report of Cowpea Mild Mottle Carlavirus on Yardlong Bean (Vigna unguiculata subsp. sesquipedalis) in Venezuela. Viruses 2012, 4, 3804-3811.

AMA Style

Brito M, Fernández-Rodríguez T, Garrido MJ, Mejías A, Romano M, Marys E. First Report of Cowpea Mild Mottle Carlavirus on Yardlong Bean (Vigna unguiculata subsp. sesquipedalis) in Venezuela. Viruses. 2012; 4(12):3804-3811.

Chicago/Turabian Style

Brito, Miriam; Fernández-Rodríguez, Thaly; Garrido, Mario J.; Mejías, Alexander; Romano, Mirtha; Marys, Edgloris. 2012. "First Report of Cowpea Mild Mottle Carlavirus on Yardlong Bean (Vigna unguiculata subsp. sesquipedalis) in Venezuela." Viruses 4, no. 12: 3804-3811.


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