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Viruses 2012, 4(12), 3804-3811; doi:10.3390/v4123804
Article

First Report of Cowpea Mild Mottle Carlavirus on Yardlong Bean (Vigna unguiculata subsp. sesquipedalis) in Venezuela

1
, 2,†, 1
, 2
, 2
 and 2,*
1 Central University of Venezuela, Agronomy Faculty, Institute of Agricultural Botany, Vegetal Virology and Phytopathogenic Bacteria Laboratory, ZIP 4579, Maracay 2101-A, Venezuela 2 Venezuelan Institute for Scientific Research (IVIC), Center for Microbiology and Cell Biology, Biotecnology and Plant Virology Laboratory, ZIP 20632, Caracas 1020-A, Venezuela Present Address: RLP AgroScience GmbH, AlPlanta-Institute for Plant Research, Breitenweg 71, D 67435 Neustadt, Germany.
* Author to whom correspondence should be addressed.
Received: 4 November 2012 / Revised: 6 December 2012 / Accepted: 11 December 2012 / Published: 14 December 2012
(This article belongs to the Section Viruses of Plants, Fungi and Protoza)
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Abstract

Yardlong bean (Vigna unguiculata subsp. sesquipedalis) plants with virus-like systemic mottling and leaf distortion were observed in both experimental and commercial fields in Aragua State, Venezuela. Symptomatic leaves were shown to contain carlavirus-like particles. RT-PCR analysis with carlavirus-specific primers was positive in all tested samples. Nucleotide sequences of the obtained amplicons showed 84%–74% similarity to corresponding sequences of Cowpea mild mottle virus (CPMMV) isolates deposited in the GenBank database. This is the first report of CPMMV in Venezuela and is thought to be the first report of CPMMV infecting yardlong bean.
Keywords: Vigna unguiculata; sesquipedalis; yardlong bean; Cowpea mild mottle carlavirus; CPMMV; diagnosis; RT-PCR; Venezuela Vigna unguiculata; sesquipedalis; yardlong bean; Cowpea mild mottle carlavirus; CPMMV; diagnosis; RT-PCR; Venezuela
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Brito, M.; Fernández-Rodríguez, T.; Garrido, M.J.; Mejías, A.; Romano, M.; Marys, E. First Report of Cowpea Mild Mottle Carlavirus on Yardlong Bean (Vigna unguiculata subsp. sesquipedalis) in Venezuela. Viruses 2012, 4, 3804-3811.

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