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Viruses 2011, 3(12), 2442-2461; doi:10.3390/v3122442

The Human Lung Adenocarcinoma Cell Line EKVX Produces an Infectious Xenotropic Murine Leukemia Virus

Laboratory of Cancer Prevention, National Cancer Institute-Frederick, Frederick, MD 21702, USA
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Received: 26 October 2011 / Revised: 22 November 2011 / Accepted: 9 December 2011 / Published: 19 December 2011
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Abstract

The cell lines of the NCI-60 panel represent different cancer types and have been widely utilized for drug screening and molecular target identification. Screening these cell lines for envelope proteins or gene sequences related to xenotropic murine leukemia viruses (X-MLVs) revealed that one cell line, EKVX, was a candidate for production of an infectious gammaretrovirus. The presence of a retrovirus infectious to human cells was confirmed by the cell-free transmission of infection to the human prostate cancer cell line LNCaP. Amplification and sequencing of additional proviral sequences from EKVX confirmed a high degree of similarity to X-MLV. The cell line EKVX was established following passage of the original tumor cells through nude mice, providing a possible source of the X-MLV found in the EKVX cells.
Keywords: EKVX; NCI-60; human cell line; xenotropic murine leukemia virus; gammaretrovirus EKVX; NCI-60; human cell line; xenotropic murine leukemia virus; gammaretrovirus
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Cmarik, J.L.; Troxler, J.A.; Hanson, C.A.; Zhang, X.; Ruscetti, S.K. The Human Lung Adenocarcinoma Cell Line EKVX Produces an Infectious Xenotropic Murine Leukemia Virus. Viruses 2011, 3, 2442-2461.

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