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Viruses 2011, 3(11), 2339-2350; doi:10.3390/v3112339

Article

The Lymantria dispar IPLB-Ld652Y Cell Line Transcriptome Comprises Diverse Virus-Associated Transcripts

Michael E. Sparks and Dawn E. Gundersen-Rindal *

USDA-ARS Invasive Insect Biocontrol and Behavior Laboratory, 10300 Baltimore Ave., Bldg 011A, Rm 214, BARC-West, Beltsville, MD 20705, USA

* Author to whom correspondence should be addressed.

Received: 30 September 2011; in revised form: 12 November 2011 / Accepted: 14 November 2011 / Published: 21 November 2011

(This article belongs to the Special Issue Insect Viruses)

Download PDF Full-Text [1097 KB, Updated Version, uploaded 25 November 2011 08:35 CET]

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Abstract: The enhanced viral susceptibility of the gypsy moth (Lymantria dispar)-derived IPLB-Ld652Y cell line has made it a popular in vitro system for studying virus-related phenomena in the Lepidoptera. Using both single-pass EST sequencing and 454-based pyrosequencing, a transcriptomic library of 14,368 putatively unique transcripts (PUTs) was produced comprising 8,476,050 high-quality, informative bases. The gene content of the IPLB-Ld652Y transcriptome was broadly assessed via comparison with the NCBI non‑redundant protein database, and more detailed functional annotation was inferred by comparison to the Swiss-Prot subset of UniProtKB. In addition to L. dispar cellular transcripts, a diverse array of both RNA and DNA virus-associated transcripts was identified within the dataset, suggestive of a high level of viral expression and activity in IPLB-Ld652Y cells. These sequence resources will provide a sound basis for developing testable experimental hypotheses by insect virologists, and suggest a number of avenues for potential research.

Keywords: RNA-Seq; EST; transcriptomics; IPLB-Ld652Y; Lepidoptera

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