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Viruses 2010, 2(3), 731-747; doi:10.3390/v2030731
Article

A Novel System for Identification of Inhibitors of Rift Valley Fever Virus Replication

1
 and
1,2,*
1 Cellular and Molecular Biology Program, University of Michigan, Ann Arbor, MI, USA 2 Department of Epidemiology, University of Michigan, Ann Arbor, MI, USA
* Author to whom correspondence should be addressed.
Received: 11 December 2009 / Revised: 7 March 2010 / Accepted: 9 March 2010 / Published: 18 March 2010

Abstract

Rift Valley fever virus (RVFV) is a human and livestock pathogen endemic to sub-Saharan Africa. We have developed a T7-dependent system for the efficient production of RVFV-like particles (RVF-VLPs) based on the virulent ZH-501 strain of RVFV. The RVF-VLPs are capable of performing a single round of infection, allowing for the study of viral replication, assembly, and infectivity. We demonstrate that these RVF-VLPs are antigenically indistinguishable from authentic RVFV and respond similarly to a wide array of known and previously unknown chemical inhibitors. This system should be useful for screening for small molecule inhibitors of RVFV replication.
Keywords: Rift Valley fever; virus-like particle; RNA virus; Bunyaviridae; Phlebovirus; minigenome; antivirals Rift Valley fever; virus-like particle; RNA virus; Bunyaviridae; Phlebovirus; minigenome; antivirals
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Piper, M.E.; Gerrard, S.R. A Novel System for Identification of Inhibitors of Rift Valley Fever Virus Replication. Viruses 2010, 2, 731-747.

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