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Viruses 2010, 2(3), 731-747; doi:10.3390/v2030731
Article

A Novel System for Identification of Inhibitors of Rift Valley Fever Virus Replication

1
 and 1,2,*
Received: 11 December 2009; in revised form: 7 March 2010 / Accepted: 9 March 2010 / Published: 18 March 2010
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Abstract: Rift Valley fever virus (RVFV) is a human and livestock pathogen endemic to sub-Saharan Africa. We have developed a T7-dependent system for the efficient production of RVFV-like particles (RVF-VLPs) based on the virulent ZH-501 strain of RVFV. The RVF-VLPs are capable of performing a single round of infection, allowing for the study of viral replication, assembly, and infectivity. We demonstrate that these RVF-VLPs are antigenically indistinguishable from authentic RVFV and respond similarly to a wide array of known and previously unknown chemical inhibitors. This system should be useful for screening for small molecule inhibitors of RVFV replication.
Keywords: Rift Valley fever; virus-like particle; RNA virus; Bunyaviridae; Phlebovirus; minigenome; antivirals Rift Valley fever; virus-like particle; RNA virus; Bunyaviridae; Phlebovirus; minigenome; antivirals
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Piper, M.E.; Gerrard, S.R. A Novel System for Identification of Inhibitors of Rift Valley Fever Virus Replication. Viruses 2010, 2, 731-747.

AMA Style

Piper ME, Gerrard SR. A Novel System for Identification of Inhibitors of Rift Valley Fever Virus Replication. Viruses. 2010; 2(3):731-747.

Chicago/Turabian Style

Piper, Mary E.; Gerrard, Sonja R. 2010. "A Novel System for Identification of Inhibitors of Rift Valley Fever Virus Replication." Viruses 2, no. 3: 731-747.



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