Viruses 2010, 2(3), 710-730; doi:10.3390/v2030710
Article

Complete Genomic Sequence of Bacteriophage Felix O1†

Jean M. Whichard 1 email, Lee A. Weigt 2 email, Douglas J. Borris 3 , Ling Ling Li 4 email, Qing Zhang 5 email, Vivek Kapur 6 email, F. William Pierson 7 email, Erika J. Lingohr 8 email, Yi-Min She 9 email, Andrew M. Kropinski 8,10 email and Nammalwar Sriranganathan 11,* email
1 Mailstop G29, Centers for Disease Control and Prevention; 1600 Clifton Road, Atlanta, GA 30329, USA 2 Smithsonian National Institution, National Museum in Natural History, MRC 534, Washington, DC 20560, USA 3 Abbot Point of Care, 185 Corkstown Road, Ottawa, ON, K2H 8V4, Canada 4 Pennsylvania State University, Department of Veterinary and Biomedical Sciences, 204 Wartick Laboratory, University Park, PA 16802 , USA 5 Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N. Seattle, WA 98109, USA 6 Pennsylvania State University, 115 Henning Bldg., University Park, PA 16802, USA 7 VA-MD Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Duck Pond Drive (0442), Blacksburg, Virginia 24061, USA 8 Public Health Agency of Canada, Laboratory for Foodborne Zoonoses, Guelph, Ontario N1G 3W4, Canada 9 Centre for Biologics Research, Health Canada, Room D159, Frederick G. Banting Building 251 Sir Frederick Banting Driveway, Tunney's Pasture, Ottawa, ON K1A 0K9, Canada 10 University of Guelph, Department of Molecular and Cellular Biology, Guelph, Ontario N1G 2W1, Canada 11 Center for Molecular Medicine and Infectious Disease; 1410 Prices Fork Road; Blacksburg, VA 24061-0342, USA
† This research was originally conducted at the Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University (Blacksburg, VA); and, the University of Minnesota, College of Veterinary Medicine, Department of Veterinary Pathobiology (St. Paul, MN).
* Author to whom correspondence should be addressed.
Received: 12 December 2009; in revised form: 25 February 2010 / Accepted: 8 March 2010 / Published: 9 March 2010
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Abstract: Bacteriophage O1 is a Myoviridae A1 group member used historically for identifying Salmonella. Sequencing revealed a single, linear, 86,155-base-pair genome with 39% average G+C content, 131 open reading frames, and 22 tRNAs. Closest protein homologs occur in Erwinia amylovora phage φEa21-4 and Escherichia coli phage wV8. Proteomic analysis indentified structural proteins: Gp23, Gp36 (major tail protein), Gp49, Gp53, Gp54, Gp55, Gp57, Gp58 (major capsid protein), Gp59, Gp63, Gp64, Gp67, Gp68, Gp69, Gp73, Gp74 and Gp77 (tail fiber). Based on phage-host codon differences, 7 tRNAs could affect translation rate during infection. Introns, holin-lysin cassettes, bacterial toxin homologs and host RNA polymerase-modifying genes were absent.
Keywords: Salmonella; bacteriophage; Myoviridae; Felix O1; DNA sequence; bioinformatics

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Cite This Article

MDPI and ACS Style

Whichard, J.M.; Weigt, L.A.; Borris, D.J.; Li, L.L.; Zhang, Q.; Kapur, V.; Pierson, F.W.; Lingohr, E.J.; She, Y.-M.; Kropinski, A.M.; Sriranganathan, N. Complete Genomic Sequence of Bacteriophage Felix O1. Viruses 2010, 2, 710-730.

AMA Style

Whichard JM, Weigt LA, Borris DJ, Li LL, Zhang Q, Kapur V, Pierson FW, Lingohr EJ, She Y-M, Kropinski AM, Sriranganathan N. Complete Genomic Sequence of Bacteriophage Felix O1. Viruses. 2010; 2(3):710-730.

Chicago/Turabian Style

Whichard, Jean M.; Weigt, Lee A.; Borris, Douglas J.; Li, Ling Ling; Zhang, Qing; Kapur, Vivek; Pierson, F. William; Lingohr, Erika J.; She, Yi-Min; Kropinski, Andrew M.; Sriranganathan, Nammalwar. 2010. "Complete Genomic Sequence of Bacteriophage Felix O1." Viruses 2, no. 3: 710-730.

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