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Viruses 2018, 10(6), 291; https://doi.org/10.3390/v10060291

Potential Application of the CRISPR/Cas9 System against Herpesvirus Infections

1
College of Life Sciences and Technology, Jinan University, Guangzhou 510632, China
2
Program in Comparative Biochemistry, University of California, Berkeley, CA 94720, USA
3
National Applied Research Laboratories, Taipei 10636, Taiwan
*
Author to whom correspondence should be addressed.
Received: 25 March 2018 / Revised: 19 May 2018 / Accepted: 19 May 2018 / Published: 29 May 2018
(This article belongs to the Special Issue Applications of CRISPR Technology in Virology 2018)
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Abstract

The CRISPR/Cas9 system has been applied in the genome editing and disruption of latent infections for herpesviruses such as the herpes simplex virus, Epstein–Barr virus, cytomegalovirus, and Kaposi’s sarcoma-associated herpesvirus. CRISPR/Cas9-directed mutagenesis can introduce similar types of mutations to the viral genome as can bacterial artificial chromosome recombination engineering, which maintains and reconstitutes the viral genome successfully. The cleavage mediated by CRISPR/Cas9 enables the manipulation of disease-associated viral strains with unprecedented efficiency and precision. Additionally, current therapies for herpesvirus productive and latent infections are limited in efficacy and cannot eradicate viruses. CRISPR/Cas9 is potentially adapted for antiviral treatment by specifically targeting viral genomes during latent infections. This review, which focuses on recently published progress, suggests that the CRISPR/Cas9 system is not only a useful tool for basic virology research, but also a promising strategy for the control and prevention of herpesvirus latent infections. View Full-Text
Keywords: CRISPR; clinical application; herpesvirus; latent infection; genome editing; HSV (Herpes Simplex Virus); EBV (Epstein–Barr Virus); CMV (cytomegalovirus); KSHV (Kaposi’s Sarcoma-Associated Herpesvirus) CRISPR; clinical application; herpesvirus; latent infection; genome editing; HSV (Herpes Simplex Virus); EBV (Epstein–Barr Virus); CMV (cytomegalovirus); KSHV (Kaposi’s Sarcoma-Associated Herpesvirus)
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Chen, Y.-C.; Sheng, J.; Trang, P.; Liu, F. Potential Application of the CRISPR/Cas9 System against Herpesvirus Infections. Viruses 2018, 10, 291.

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