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Viruses 2018, 10(5), 245; https://doi.org/10.3390/v10050245

Transposition Behavior Revealed by High-Resolution Description of Pseudomonas Aeruginosa Saltovirus Integration Sites

1
Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Université Paris-Saclay, 91198 Gif-sur-Yvette CEDEX, France
2
I. I. Mechnikov Research Institute for Vaccines & Sera, Moscow 105064, Russia
*
Author to whom correspondence should be addressed.
Received: 9 April 2018 / Revised: 27 April 2018 / Accepted: 4 May 2018 / Published: 7 May 2018
(This article belongs to the Special Issue Bacteriophage Genomes and Genomics: News from the Wild)
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Abstract

Transposable phages, also called saltoviruses, of which the Escherichia coli phage Mu is the reference, are temperate phages that multiply their genome through replicative transposition at multiple sites in their host chromosome. The viral genome is packaged together with host DNA at both ends. In the present work, genome sequencing of three Pseudomonas aeruginosa transposable phages, HW12, 2P1, and Ab30, incidentally gave us access to the location of thousands of replicative integration sites and revealed the existence of a variable number of hotspots. Taking advantage of deep sequencing, we then designed an experiment to study 13,000,000 transposon integration sites of bacteriophage Ab30. The investigation revealed the presence of 42 transposition hotspots adjacent to bacterial interspersed mosaic elements (BIME) accounting for 5% of all transposition sites. The rest of the sites appeared widely distributed with the exception of coldspots associated with low G-C content segments, including the putative O-antigen biosynthesis cluster. Surprisingly, 0.4% of the transposition events occurred in a copy of the phage genome itself, indicating that the previously described immunity against such events is slightly leaky. This observation allowed drawing an image of the phage chromosome supercoiling into four loops. View Full-Text
Keywords: transposable phages; transposon integration; hotspots; deep sequencing; chromosomal domain; supercoiling; imaging transposable phages; transposon integration; hotspots; deep sequencing; chromosomal domain; supercoiling; imaging
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Vergnaud, G.; Midoux, C.; Blouin, Y.; Bourkaltseva, M.; Krylov, V.; Pourcel, C. Transposition Behavior Revealed by High-Resolution Description of Pseudomonas Aeruginosa Saltovirus Integration Sites. Viruses 2018, 10, 245.

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