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Viruses 2018, 10(5), 244; https://doi.org/10.3390/v10050244

An Ointment Consisting of the Phage Lysin LysGH15 and Apigenin for Decolonization of Methicillin-Resistant Staphylococcus aureus from Skin Wounds

1
Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Xi’an Road 5333#, Changchun 130062, China
2
Animal Science and Technology College, Jilin Agricultural University, Changchun 130117, China
3
First Hospital of Jilin University, Jilin University, Changchun 130021, China
4
Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonose, Yangzhou University, Yangzhou 225009, China
These authors contribute equally to this manuscript.
*
Author to whom correspondence should be addressed.
Received: 16 March 2018 / Revised: 3 May 2018 / Accepted: 4 May 2018 / Published: 6 May 2018
(This article belongs to the Special Issue Phage Lytic Enzymes and Their Applications)
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Abstract

Staphylococcus aureus (S. aureus) is a common and dangerous pathogen that causes various infectious diseases. Skin damage, such as burn wounds, are at high risk of Staphylococcus aureus colonization and infection, which increases morbidity and mortality. The phage lysin LysGH15 exhibits highly efficient lytic activity against methicillin-resistant S. aureus (MRSA) and methicillin-susceptible S. aureus (MSSA) strains. Apigenin (api) significantly decreases haemolysis of rabbit erythrocytes caused by S. aureus and shows anti-inflammatory function. LysGH15 and api were added to Aquaphor to form an LysGH15-api-Aquaphor (LAA) ointment. The LAA ointment simultaneously exhibited bactericidal activity against S. aureus and inhibited haemolysis. In an LAA-treated mouse model of an MRSA-infected skin wound, the mean bacterial colony count decreased to approximately 102 CFU/mg at 18 h after treatment (and the bacteria became undetectable at 96 h), whereas the mean count in untreated mice was approximately 105 CFU/mg of tissue. The LAA ointment also reduced the levels of pro-inflammatory cytokines (TNF-α, IL-1β, and IFN-γ) and accelerated wound healing in the mouse model. These data demonstrate the potential efficacy of a combination of LysGH15 and api for use as a topical antimicrobial agent against S. aureus. View Full-Text
Keywords: phage lysin; apigenin; ointment; methicillin-resistant Staphylococcus aureus; skin infection phage lysin; apigenin; ointment; methicillin-resistant Staphylococcus aureus; skin infection
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Cheng, M.; Zhang, L.; Zhang, H.; Li, X.; Wang, Y.; Xia, F.; Wang, B.; Cai, R.; Guo, Z.; Zhang, Y.; Ji, Y.; Sun, C.; Feng, X.; Lei, L.; Yang, Y.; Han, W.; Gu, J. An Ointment Consisting of the Phage Lysin LysGH15 and Apigenin for Decolonization of Methicillin-Resistant Staphylococcus aureus from Skin Wounds. Viruses 2018, 10, 244.

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