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Viruses 2018, 10(4), 173; https://doi.org/10.3390/v10040173

Vitamin E Supplementation Ameliorates Newcastle Disease Virus-Induced Oxidative Stress and Alleviates Tissue Damage in the Brains of Chickens

1
Shanghai Veterinary Research Institute (SHVRI), Chinese Academy of Agricultural Sciences (CAAS), Shanghai 200241, China
2
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun 130062, China
3
Biomedical and Life Sciences, Lancaster University, Lancaster LA1 4YG, UK
4
Avian Viral Diseases Programme, The Pirbright Institute, Surrey GU24 0NF, UK
5
Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China
*
Authors to whom correspondence should be addressed.
Received: 13 February 2018 / Revised: 26 March 2018 / Accepted: 31 March 2018 / Published: 3 April 2018
(This article belongs to the Section Animal Viruses)
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Abstract

Newcastle disease (ND), characterized by visceral, respiratory, and neurological pathologies, causes heavy economic loss in the poultry industry around the globe. While significant advances have been made in effective diagnosis and vaccine development, molecular mechanisms of ND virus (NDV)-induced neuropathologies remain elusive. In this study, we report the magnitude of oxidative stress and histopathological changes induced by the virulent NDV (ZJ1 strain) and assess the impact of vitamin E in alleviating these pathologies. Comparative profiling of plasma and brains from mock and NDV-infected chicken demonstrated alterations in several oxidative stress makers such as nitric oxide, glutathione, malondialdehyde, total antioxidant capacity, glutathione S-transferase, superoxide dismutase, and catalases. While decreased levels of glutathione and total antioxidant capacity and increased concentrations of malondialdehyde and nitric oxide were observed in NDV-challenged birds at all time points, these alterations were eminent at latter time points (5 days post infection). Additionally, significant decreases in the activities of glutathione S-transferase, superoxide dismutase, and catalase were observed in the plasma and brains collected from NDV-infected chickens. Intriguingly, we observed that supplementation of vitamin E can significantly reduce the alteration of oxidative stress parameters. Under NDV infection, extensive histopathological alterations were observed in chicken brain including neural inflammation, capillary hyperemia, necrosis, and loss of prominent axons, which were reduced with the treatment of vitamin E. Taken together, our findings highlight that neurotropic NDV induces extensive tissue damage in the brain and alters plasma oxidative stress profiles. These findings also demonstrate that supplementing vitamin E ameliorates these pathologies in chickens and proposes its supplementation for NDV-induced stresses. View Full-Text
Keywords: Newcastle disease; oxidative stress; histopathology; vitamin E; brain; plasma; glutathione; malondialdehyde; nitric oxide; superoxide dismutase Newcastle disease; oxidative stress; histopathology; vitamin E; brain; plasma; glutathione; malondialdehyde; nitric oxide; superoxide dismutase
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Rehman, Z.U.; Qiu, X.; Sun, Y.; Liao, Y.; Tan, L.; Song, C.; Yu, S.; Ding, Z.; Munir, M.; Nair, V.; Meng, C.; Ding, C. Vitamin E Supplementation Ameliorates Newcastle Disease Virus-Induced Oxidative Stress and Alleviates Tissue Damage in the Brains of Chickens. Viruses 2018, 10, 173.

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