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Forests 2017, 8(12), 501; doi:10.3390/f8120501

Combined Analysis of mRNAs and miRNAs to Identify Genes Related to Biological Characteristics of Autotetraploid Paulownia

1
Institute of Paulownia, Henan Agricultural University, 95 Wenhua Road, Jinshui District, Zhengzhou 450002, Henan, China
2
College of Forestry, Henan Agricultural University, 95 Wenhua Road, Jinshui District, Zhengzhou 450002, Henan, China
3
Forestry Academy of Henan, Zhengzhou 450002, Henan, China
*
Author to whom correspondence should be addressed.
Received: 20 September 2017 / Revised: 5 December 2017 / Accepted: 8 December 2017 / Published: 13 December 2017
(This article belongs to the Section Forest Ecophysiology and Biology)
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Abstract

Autopolyploid plants and their organs are larger than their corresponding diploid ancestors, and they attract considerable attention for plant breeding. Paulownia is a fast-growing tree. To identify genes related to the biological characteristics of tetraploid Paulownia, transcriptome and small RNA sequencing were used to identify the key gene expression regulation in tetraploid Paulownia fortunei and tetraploid P. tomentosa and their corresponding diploids. A total of 1977 common differentially expressed genes (DEGs) and 89 differentially expressed miRNAs (DEMs) (38 conserved and 51 novel) were obtained in tetraploid vs. diploid comparisons of the two Paulownia species, and 18 target genes were identified by target prediction. Finally, by analyzing the expression profiles of the DEGs and DEMs and their target genes, we discovered that Pau-miR169, Pau-miR408 and Pau-miR156 interacted with their target gene nuclear transcription factor Y subunit A-9 (NF-YA9), serine/threonine protein phosphatase (PP1) and s-adenosyl-methionine-sterol-c-methyltransfera—se (SAM:SMT) to regulate the abiotic stress tolerance and the timber quality of the tetraploid Paulownia. This study lays a molecular biology foundation for understanding autotetraploid Paulownia and will benefit future breeding work. View Full-Text
Keywords: Autopolyploid Paulownia; transcriptome sequencing; miRNA sequencing; gene expression analysis Autopolyploid Paulownia; transcriptome sequencing; miRNA sequencing; gene expression analysis
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Cao, X.; Xu, E.; Zhai, X.; Dong, Y.; Fan, G. Combined Analysis of mRNAs and miRNAs to Identify Genes Related to Biological Characteristics of Autotetraploid Paulownia. Forests 2017, 8, 501.

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