Immobilization of Trypsin in Lignocellulosic Waste Material to Produce Peptides with Bioactive Potential from Whey Protein
AbstractIn this study, trypsin (Enzyme Comission 18.104.22.168) was immobilized in a low cost, lignocellulosic support (corn cob powder—CCP) with the goal of obtaining peptides with bioactive potential from cheese whey. The pretreated support was activated with glyoxyl groups, glutaraldehyde and IDA-glyoxyl. The immobilization yields of the derivatives were higher than 83%, and the retention of catalytic activity was higher than 74%. The trypsin-glyoxyl-CCP derivative was thermally stable at 65 °C, a value that was 1090-fold higher than that obtained with the free enzyme. The trypsin-IDA-glyoxyl-CCP and trypsin-glutaraldehyde-CCP derivatives had thermal stabilities that were 883- and five-fold higher, respectively, then those obtained with the free enzyme. In the batch experiments, trypsin-IDA-glyoxyl-CCP retained 91% of its activity and had a degree of hydrolysis of 12.49%, while the values for trypsin-glyoxyl-CCP were 87% and 15.46%, respectively. The stabilized derivative trypsin-glyoxyl-CCP was also tested in an upflow packed-bed reactor. The hydrodynamic characterization of this reactor was a plug flow pattern, and the kinetics of this system provided a relative activity of 3.04 ± 0.01 U·g−1 and an average degree of hydrolysis of 23%, which were suitable for the production of potentially bioactive peptides. View Full-Text
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Bassan, J.C.; de Souza Bezerra, T.M.; Peixoto, G.; da Cruz, C.Z.P.; Galán, J.P.M.; Vaz, A.B.S.; Garrido, S.S.; Filice, M.; Monti, R. Immobilization of Trypsin in Lignocellulosic Waste Material to Produce Peptides with Bioactive Potential from Whey Protein. Materials 2016, 9, 357.
Bassan JC, de Souza Bezerra TM, Peixoto G, da Cruz CZP, Galán JPM, Vaz ABS, Garrido SS, Filice M, Monti R. Immobilization of Trypsin in Lignocellulosic Waste Material to Produce Peptides with Bioactive Potential from Whey Protein. Materials. 2016; 9(5):357.Chicago/Turabian Style
Bassan, Juliana C.; de Souza Bezerra, Thaís M.; Peixoto, Guilherme; da Cruz, Clariana Z.P.; Galán, Julián P.M.; Vaz, Aline B.S.; Garrido, Saulo S.; Filice, Marco; Monti, Rubens. 2016. "Immobilization of Trypsin in Lignocellulosic Waste Material to Produce Peptides with Bioactive Potential from Whey Protein." Materials 9, no. 5: 357.
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