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Materials 2015, 8(8), 4710-4719; doi:10.3390/ma8084710

Study on Electrochemical Insulin Sensing Utilizing a DNA Aptamer-Immobilized Gold Electrode

Graduate School of Engineering, Soka University, 1-236 Tangi, Hachioji, Tokyo, 192-8577, Japan
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Author to whom correspondence should be addressed.
Academic Editor: Jun-ichi Anzai
Received: 30 June 2015 / Revised: 14 July 2015 / Accepted: 21 July 2015 / Published: 24 July 2015
View Full-Text   |   Download PDF [433 KB, uploaded 24 July 2015]   |  

Abstract

We investigated an insulin-sensing method by utilizing an insulin-binding aptamer IGA3, which forms an anti-parallel G-quadruplex with folded single strands. Spectroscopic observation indicates that some anti-parallel G-quadruplex bind hemin and show peroxidase activity. In this study, the peroxidase activity of IGA3 with hemin was confirmed by spectrophotometric measurements, i.e., the activity was three-times higher than hemin itself. IGA3 was then immobilized onto a gold electrode to determine its electrochemical activity. The peroxidase activity of the immobilized IGA3-hemin complex was determined by cyclic voltammetry, and a cathodic peak current of the electrode showed a dependence on the concentration of H2O2. The cathodic peak current of the IGA3-hemin complex decreased by binding it to insulin, and this decrease depended on the concentration of insulin. View Full-Text
Keywords: DNA aptamer; hemin; insulin; glucagon; G-quadruplex; peroxidase activity; gold electrode DNA aptamer; hemin; insulin; glucagon; G-quadruplex; peroxidase activity; gold electrode
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Kubo, I.; Eguchi, T. Study on Electrochemical Insulin Sensing Utilizing a DNA Aptamer-Immobilized Gold Electrode. Materials 2015, 8, 4710-4719.

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