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Materials 2015, 8(6), 3584-3606; doi:10.3390/ma8063584

Biomimetic Coating on Porous Alumina for Tissue Engineering: Characterisation by Cell Culture and Confocal Microscopy

Biomedical Engineering, AMME J07, University of Sydney, Sydney, NSW 2006, Australia
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Author to whom correspondence should be addressed.
Academic Editor: Naozumi Teramoto
Received: 9 March 2015 / Revised: 26 May 2015 / Accepted: 2 June 2015 / Published: 17 June 2015
(This article belongs to the Special Issue Bioceramics)

Abstract

In this study porous alumina samples were prepared and then coated using the biomimetic coating technique using a five times Simulated Body Fluid (5.0SBF) as the growth solution. A coating was achieved after pre-treatment with concentrated acid. From elemental analysis, the coating contained calcium and phosphorous, but also sodium and chlorine. Halite was identified by XRD, a sodium chloride phase. Sintering was done to remove the halite phase. Once halite was burnt off, the calcium phosphate crystals were not covered with halite and, therefore, the apatite phases can be clearly observed. Cell culturing showed sufficient cell attachment to the less porous alumina, Sample B, that has more calcium phosphate growth, while the porous alumina, Sample A, with minimal calcium phosphate growth attained very little cell attachment. This is likely due to the contribution that calcium phosphate plays in the attachment of bone-like cells to a bioinert ceramic such as alumina. These results were repeated on both SEM and confocal microscopy analysis. Confocal microscopy was a novel characterisation approach which gave useful information and was a visual aid. View Full-Text
Keywords: tissue engineering; porous alumina; biomimetic coating; calcium phosphate; confocal microscopy tissue engineering; porous alumina; biomimetic coating; calcium phosphate; confocal microscopy
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Kolos, E.; Ruys, A.J. Biomimetic Coating on Porous Alumina for Tissue Engineering: Characterisation by Cell Culture and Confocal Microscopy. Materials 2015, 8, 3584-3606.

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