Next Article in Journal
The Effects of Zr Doping on the Optical, Electrical and Microstructural Properties of Thin ZnO Films Deposited by Atomic Layer Deposition
Next Article in Special Issue
Active Nanomaterials to Meet the Challenge of Dental Pulp Regeneration
Previous Article in Journal
Simplified Calculation Model and Experimental Study of Latticed Concrete-Gypsum Composite Panels
Previous Article in Special Issue
Integrating Microtissues in Nanofiber Scaffolds for Regenerative Nanomedicine
Article Menu

Export Article

Open AccessArticle
Materials 2015, 8(10), 7217-7229; doi:10.3390/ma8105376

Active Nanofibrous Membrane Effects on Gingival Cell Inflammatory Response

1
INSERM (French National Institute of Health and Medical Research), UMR 1109, Osteoarticular and Dental Regenerative Nanomedicine laboratory, Faculté de Médecine, FMTS, Strasbourg Cedex F-67085, France
2
Department of Periodontology, University of Strasbourg, Dental Faculty, 8 rue Sainte-Elisabeth, Strasbourg F-67000, France
*
Author to whom correspondence should be addressed.
Academic Editor: Marco Salerno
Received: 25 August 2015 / Revised: 8 October 2015 / Accepted: 20 October 2015 / Published: 27 October 2015
(This article belongs to the Special Issue Therapeutics Delivery Systems for Regenerative Nanomedicine)
View Full-Text   |   Download PDF [10118 KB, uploaded 27 October 2015]   |  

Abstract

Alpha-melanocyte stimulating hormone (α-MSH) is involved in normal skin wound healing and also has anti-inflammatory properties. The association of α-MSH to polyelectrolyte layers with various supports has been shown to improve these anti-inflammatory properties. This study aimed to evaluate the effects of nanofibrous membrane functionalized with α-MSH linked to polyelectrolyte layers on gingival cell inflammatory response. Human oral epithelial cells (EC) and fibroblasts (FB) were cultured on plastic or electrospun Poly-#-caprolactone (PCL) membranes with α-MSH covalently coupled to Poly-L-glutamic acid (PGA-α-MSH), for 6 to 24 h. Cells were incubated with or without Porphyromonas gingivalis lipopolysaccharide (Pg-LPS). Cell proliferation and migration were determined using AlamarBlue test and scratch assay. Expression of interleukin-6 (IL-6), tumor necrosis factor (TNF-α), and transforming growth factor-beta (TGF-β) was evaluated using RT-qPCR method. Cell cultures on plastic showed that PGA-α-MSH reduced EC and FB migration and decreased IL-6 and TGF-β expression in Pg-LPS stimulated EC. PGA-α-MSH functionalized PCL membranes reduced proliferation of Pg-LPS stimulated EC and FB. A significant decrease of IL-6, TNF-α, and TGF-β expression was also observed in Pg-LPS stimulated EC and FB. This study showed that the functionalization of nanofibrous PCL membranes efficiently amplified the anti-inflammatory effect of PGA-α-MSH on gingival cells. View Full-Text
Keywords: anti-inflammatory agents; lipopolysaccharides; fibroblasts; epithelial cells; polycaprolactone; cytokines anti-inflammatory agents; lipopolysaccharides; fibroblasts; epithelial cells; polycaprolactone; cytokines
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Morand, D.-N.; Huck, O.; Keller, L.; Jessel, N.; Tenenbaum, H.; Davideau, J.-L. Active Nanofibrous Membrane Effects on Gingival Cell Inflammatory Response. Materials 2015, 8, 7217-7229.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Materials EISSN 1996-1944 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top