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Materials 2014, 7(4), 2490-2500; doi:10.3390/ma7042490
Article

Field Effect Transistor Biosensor Using Antigen Binding Fragment for Detecting Tumor Marker in Human Serum

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Received: 3 February 2014; in revised form: 15 March 2014 / Accepted: 19 March 2014 / Published: 27 March 2014
(This article belongs to the Special Issue Advanced Nanomaterials for Biosensors)
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Abstract: Detection of tumor markers is important for cancer diagnosis. Field-effect transistors (FETs) are a promising method for the label-free detection of trace amounts of biomolecules. However, detection of electrically charged proteins using antibody-immobilized FETs is limited by ionic screening by the large probe molecules adsorbed to the transistor gate surface, reducing sensor responsiveness. Here, we investigated the effect of probe molecule size on the detection of a tumor marker, α-fetoprotein (AFP) using a FET biosensor. We demonstrated that the small receptor antigen binding fragment (Fab), immobilized on a sensing surface as small as 2–3 nm, offers a higher degree of sensitivity and a wider concentration range (100 pg/mL–1 μg/mL) for the FET detection of AFP in buffer solution, compared to the whole antibody. Therefore, the use of a small Fab probe molecule instead of a whole antibody is shown to be effective for improving the sensitivity of AFP detection in FET biosensors. Furthermore, we also demonstrated that a Fab-immobilized FET subjected to a blocking treatment, to avoid non-specific interactions, could sensitively and selectively detect AFP in human serum.
Keywords: antigen binding fragment; label-free detection; field effect transistor; immunosensor; Langmuir-type adsorption model antigen binding fragment; label-free detection; field effect transistor; immunosensor; Langmuir-type adsorption model
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Cheng, S.; Hotani, K.; Hideshima, S.; Kuroiwa, S.; Nakanishi, T.; Hashimoto, M.; Mori, Y.; Osaka, T. Field Effect Transistor Biosensor Using Antigen Binding Fragment for Detecting Tumor Marker in Human Serum. Materials 2014, 7, 2490-2500.

AMA Style

Cheng S, Hotani K, Hideshima S, Kuroiwa S, Nakanishi T, Hashimoto M, Mori Y, Osaka T. Field Effect Transistor Biosensor Using Antigen Binding Fragment for Detecting Tumor Marker in Human Serum. Materials. 2014; 7(4):2490-2500.

Chicago/Turabian Style

Cheng, Shanshan; Hotani, Kaori; Hideshima, Sho; Kuroiwa, Shigeki; Nakanishi, Takuya; Hashimoto, Masahiro; Mori, Yasuro; Osaka, Tetsuya. 2014. "Field Effect Transistor Biosensor Using Antigen Binding Fragment for Detecting Tumor Marker in Human Serum." Materials 7, no. 4: 2490-2500.


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