Materials 2013, 6(12), 5690-5699; doi:10.3390/ma6125690
Communication

Magnetic Fe3O4-Based Sandwich-Type Biosensor Using Modified Gold Nanoparticles as Colorimetric Probes for the Detection of Dopamine

College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, Henan, China
* Authors to whom correspondence should be addressed.
Received: 12 November 2013; in revised form: 27 November 2013 / Accepted: 2 December 2013 / Published: 5 December 2013
(This article belongs to the Special Issue Advanced Nanomaterials for Biosensors)
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Abstract: In this work, we designed a visual biosensor for dopamine (DA) detection using magnetic Fe3O4 particles and dithiobis(sulfosuccinimidylpropionate)-modified gold nanoparticles (DTSSP-AuNPs) as the recognition elements. Specifically, DA molecules were assembled onto the surface of DTSSP-AuNPs via the amine coupling reaction between the amino group of DA and activated carboxyl group of DTSSP. Accordingly, DA-anchored DTSSP-AuNPs were captured by Fe3O4 through the interaction of catechol and iron. In a magnetic field, the formed Fe3O4-DA-DTSSP-AuNPs conjugates were easily removed from the solution, leading to fading of the AuNPs suspension and decrease of the UV/Vis signal. As a result, a detection limit of 10 nM for DA was achieved. The theoretical simplicity and high selectivity demonstrated that the sandwich-type strategy based on Fe3O4 and AuNPs would lead to many colorimetric detection applications in clinical study by rationally designing the surface chemistry of AuNPs and Fe3O4.
Keywords: gold nanoparticles; magnetic particle; biosensor; dopamine; colorimetric assay

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MDPI and ACS Style

Wang, Z.; Bai, Y.; Wei, W.; Xia, N.; Du, Y. Magnetic Fe3O4-Based Sandwich-Type Biosensor Using Modified Gold Nanoparticles as Colorimetric Probes for the Detection of Dopamine. Materials 2013, 6, 5690-5699.

AMA Style

Wang Z, Bai Y, Wei W, Xia N, Du Y. Magnetic Fe3O4-Based Sandwich-Type Biosensor Using Modified Gold Nanoparticles as Colorimetric Probes for the Detection of Dopamine. Materials. 2013; 6(12):5690-5699.

Chicago/Turabian Style

Wang, Zhiyong; Bai, Yanyan; Wei, Wenchao; Xia, Ning; Du, Yuhui. 2013. "Magnetic Fe3O4-Based Sandwich-Type Biosensor Using Modified Gold Nanoparticles as Colorimetric Probes for the Detection of Dopamine." Materials 6, no. 12: 5690-5699.

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