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Materials 2013, 6(12), 5690-5699; doi:10.3390/ma6125690
Communication

Magnetic Fe3O4-Based Sandwich-Type Biosensor Using Modified Gold Nanoparticles as Colorimetric Probes for the Detection of Dopamine

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Received: 12 November 2013; in revised form: 27 November 2013 / Accepted: 2 December 2013 / Published: 5 December 2013
(This article belongs to the Special Issue Advanced Nanomaterials for Biosensors)
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Abstract: In this work, we designed a visual biosensor for dopamine (DA) detection using magnetic Fe3O4 particles and dithiobis(sulfosuccinimidylpropionate)-modified gold nanoparticles (DTSSP-AuNPs) as the recognition elements. Specifically, DA molecules were assembled onto the surface of DTSSP-AuNPs via the amine coupling reaction between the amino group of DA and activated carboxyl group of DTSSP. Accordingly, DA-anchored DTSSP-AuNPs were captured by Fe3O4 through the interaction of catechol and iron. In a magnetic field, the formed Fe3O4-DA-DTSSP-AuNPs conjugates were easily removed from the solution, leading to fading of the AuNPs suspension and decrease of the UV/Vis signal. As a result, a detection limit of 10 nM for DA was achieved. The theoretical simplicity and high selectivity demonstrated that the sandwich-type strategy based on Fe3O4 and AuNPs would lead to many colorimetric detection applications in clinical study by rationally designing the surface chemistry of AuNPs and Fe3O4.
Keywords: gold nanoparticles; magnetic particle; biosensor; dopamine; colorimetric assay gold nanoparticles; magnetic particle; biosensor; dopamine; colorimetric assay
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Wang, Z.; Bai, Y.; Wei, W.; Xia, N.; Du, Y. Magnetic Fe3O4-Based Sandwich-Type Biosensor Using Modified Gold Nanoparticles as Colorimetric Probes for the Detection of Dopamine. Materials 2013, 6, 5690-5699.

AMA Style

Wang Z, Bai Y, Wei W, Xia N, Du Y. Magnetic Fe3O4-Based Sandwich-Type Biosensor Using Modified Gold Nanoparticles as Colorimetric Probes for the Detection of Dopamine. Materials. 2013; 6(12):5690-5699.

Chicago/Turabian Style

Wang, Zhiyong; Bai, Yanyan; Wei, Wenchao; Xia, Ning; Du, Yuhui. 2013. "Magnetic Fe3O4-Based Sandwich-Type Biosensor Using Modified Gold Nanoparticles as Colorimetric Probes for the Detection of Dopamine." Materials 6, no. 12: 5690-5699.


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