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Materials 2014, 7(2), 1342-1359; doi:10.3390/ma7021342
Article

Gelatin-Based Hydrogels Promote Chondrogenic Differentiation of Human Adipose Tissue-Derived Mesenchymal Stem Cells In Vitro

1,†,* , 2,†,* , 2
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1 Department of Cell Biology, Rostock University Medical Center, Schillingallee 69, Rostock D-18057, Germany 2 Polymer Chemistry and Biomaterials Group, Gent University, Krijgslaan 281, Building S4, Gent BE-9000, Belgium 3 DOT GmbH, Charles-Darwin-Ring 1a, Rostock D-18059, Germany 4 NMR and Structure Analysis Research Group, Gent University, Krijgslaan 281, Building S4, Gent BE-9000, Belgium These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 20 December 2013 / Revised: 24 January 2014 / Accepted: 27 January 2014 / Published: 19 February 2014
(This article belongs to the Special Issue Biocompatibility of Materials 2013)
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Abstract

Due to the weak regeneration potential of cartilage, there is a high clinical incidence of articular joint disease, leading to a strong demand for cartilaginous tissue surrogates. The aim of this study was to evaluate a gelatin-based hydrogel for its suitability to support chondrogenic differentiation of human mesenchymal stem cells. Gelatin-based hydrogels are biodegradable, show high biocompatibility, and offer possibilities to introduce functional groups and/or ligands. In order to prove their chondrogenesis-supporting potential, a hydrogel film was developed and compared with standard cell culture polystyrene regarding the differentiation behavior of human mesenchymal stem cells. Cellular basis for this study were human adipose tissue-derived mesenchymal stem cells, which exhibit differentiation potential along the adipogenic, osteogenic and chondrogenic lineage. The results obtained show a promotive effect of gelatin-based hydrogels on chondrogenic differentiation of mesenchymal stem cells in vitro and therefore encourage subsequent in vivo studies.
Keywords: adipose tissue-derived mesenchymal stem cells (adMSC); gelatin-based hydrogels; chondrogenic differentiation; tissue engineering adipose tissue-derived mesenchymal stem cells (adMSC); gelatin-based hydrogels; chondrogenic differentiation; tissue engineering
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Salamon, A.; van Vlierberghe, S.; van Nieuwenhove, I.; Baudisch, F.; Graulus, G.-J.; Benecke, V.; Alberti, K.; Neumann, H.-G.; Rychly, J.; Martins, J.C.; Dubruel, P.; Peters, K. Gelatin-Based Hydrogels Promote Chondrogenic Differentiation of Human Adipose Tissue-Derived Mesenchymal Stem Cells In Vitro. Materials 2014, 7, 1342-1359.

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