Materials 2014, 7(2), 1342-1359; doi:10.3390/ma7021342
Article

Gelatin-Based Hydrogels Promote Chondrogenic Differentiation of Human Adipose Tissue-Derived Mesenchymal Stem Cells In Vitro

1,†,* email, 2,†,* email, 2email, 1email, 2email, 1email, 1, 3email, 1email, 4email, 2email and 1email
Received: 20 December 2013; in revised form: 24 January 2014 / Accepted: 27 January 2014 / Published: 19 February 2014
(This article belongs to the Special Issue Biocompatibility of Materials 2013)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Due to the weak regeneration potential of cartilage, there is a high clinical incidence of articular joint disease, leading to a strong demand for cartilaginous tissue surrogates. The aim of this study was to evaluate a gelatin-based hydrogel for its suitability to support chondrogenic differentiation of human mesenchymal stem cells. Gelatin-based hydrogels are biodegradable, show high biocompatibility, and offer possibilities to introduce functional groups and/or ligands. In order to prove their chondrogenesis-supporting potential, a hydrogel film was developed and compared with standard cell culture polystyrene regarding the differentiation behavior of human mesenchymal stem cells. Cellular basis for this study were human adipose tissue-derived mesenchymal stem cells, which exhibit differentiation potential along the adipogenic, osteogenic and chondrogenic lineage. The results obtained show a promotive effect of gelatin-based hydrogels on chondrogenic differentiation of mesenchymal stem cells in vitro and therefore encourage subsequent in vivo studies.
Keywords: adipose tissue-derived mesenchymal stem cells (adMSC); gelatin-based hydrogels; chondrogenic differentiation; tissue engineering
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MDPI and ACS Style

Salamon, A.; van Vlierberghe, S.; van Nieuwenhove, I.; Baudisch, F.; Graulus, G.-J.; Benecke, V.; Alberti, K.; Neumann, H.-G.; Rychly, J.; Martins, J.C.; Dubruel, P.; Peters, K. Gelatin-Based Hydrogels Promote Chondrogenic Differentiation of Human Adipose Tissue-Derived Mesenchymal Stem Cells In Vitro. Materials 2014, 7, 1342-1359.

AMA Style

Salamon A, van Vlierberghe S, van Nieuwenhove I, Baudisch F, Graulus G-J, Benecke V, Alberti K, Neumann H-G, Rychly J, Martins JC, Dubruel P, Peters K. Gelatin-Based Hydrogels Promote Chondrogenic Differentiation of Human Adipose Tissue-Derived Mesenchymal Stem Cells In Vitro. Materials. 2014; 7(2):1342-1359.

Chicago/Turabian Style

Salamon, Achim; van Vlierberghe, Sandra; van Nieuwenhove, Ine; Baudisch, Frank; Graulus, Geert-Jan; Benecke, Verena; Alberti, Kristin; Neumann, Hans-Georg; Rychly, Joachim; Martins, José C.; Dubruel, Peter; Peters, Kirsten. 2014. "Gelatin-Based Hydrogels Promote Chondrogenic Differentiation of Human Adipose Tissue-Derived Mesenchymal Stem Cells In Vitro." Materials 7, no. 2: 1342-1359.


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