Materials 2014, 7(2), 1097-1112; doi:10.3390/ma7021097
Article

The Effect of Exogenous Zinc Concentration on the Responsiveness of MC3T3-E1 Pre-Osteoblasts to Surface Microtopography: Part II (Differentiation)

Received: 20 December 2013; in revised form: 24 January 2014 / Accepted: 28 January 2014 / Published: 11 February 2014
(This article belongs to the Special Issue Biocompatibility of Materials 2013)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Osseointegration of bone implants is a vital part of the recovery process. Numerous studies have shown that micropatterned geometries can promote cell-substrate associations and strengthen the bond between tissue and the implanted material. As demonstrated previously, exogenous zinc levels can influence the responsiveness of pre-osteoblasts to micropatterns and modify their migratory behavior. In this study, we sought to determine the effect of exogenous zinc on differentiation of osteoblasts cultured on micropatterned vs. planar substrates. Levels of activated metalloproteinase-2 (MMP-2) and transforming growth factor-beta 1 (TGF-β1), as well as early stage differentiation marker alkaline phosphatase, were altered with the addition of zinc. These results suggest that exogenous zinc concentration and micropatterning may interdependently modulate osteoblast differentiation.
Keywords: osteoblast; zinc; micropatterns; transforming growth factor-beta 1; metalloproteinase-2
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MDPI and ACS Style

Dorst, K.; Rammelkamp, D.; Hadjiargyrou, M.; Meng, Y. The Effect of Exogenous Zinc Concentration on the Responsiveness of MC3T3-E1 Pre-Osteoblasts to Surface Microtopography: Part II (Differentiation). Materials 2014, 7, 1097-1112.

AMA Style

Dorst K, Rammelkamp D, Hadjiargyrou M, Meng Y. The Effect of Exogenous Zinc Concentration on the Responsiveness of MC3T3-E1 Pre-Osteoblasts to Surface Microtopography: Part II (Differentiation). Materials. 2014; 7(2):1097-1112.

Chicago/Turabian Style

Dorst, Kathryn; Rammelkamp, Derek; Hadjiargyrou, Michael; Meng, Yizhi. 2014. "The Effect of Exogenous Zinc Concentration on the Responsiveness of MC3T3-E1 Pre-Osteoblasts to Surface Microtopography: Part II (Differentiation)." Materials 7, no. 2: 1097-1112.

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