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Materials 2014, 7(2), 1142-1154; doi:10.3390/ma7021142
Article

Carbon Felt-Based Bioelectrocatalytic Flow-Through Detectors: 2,6-Dichlorophenol Indophenol and Peroxidase Coadsorbed Carbon-Felt for Flow-Amperometric Determination of Hydrogen Peroxide

1
 and 2,*
Received: 30 November 2013; in revised form: 8 January 2014 / Accepted: 7 February 2014 / Published: 12 February 2014
(This article belongs to the Special Issue Functional Materials and Proteins for Bio-Sensing Applications)
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Abstract: 2,6-dichlorophenol indophenol (DCIP) and horseradish peroxidase (HRP) were coadsorbed on a porous carbon felt (CF) from their mixed aqueous solution under ultrasound irradiation for 5 min. The resulting DCIP and HRP-coadsorbed CF (DCIP/HRP-CF) showed an excellent bioelectrocatalytic activity for the reduction of H2O2. The coadsorption of DCIP together with HRP was essential to obtain larger bioelectrocatalytic current to H2O2. The DCIP/HRP-CF was successfully used as a working electrode unit of a bioelectrocatalytic flow-through detector for highly sensitive and continuous amperometric determination of H2O2. Under the optimized operational conditions (i.e., applied potential, +0.2 V versus Ag/AgCl; carrier pH 5.0, and carrier flow rate, 1.9 mL/min), the cathodic peak current of H2O2 linearly increased over the concentration range from 0.1 to 30 µM (the sensitivity, 0.88 µA/µM (slope of linear part); the limit of detection, 0.1 µM (S/N = 3) current noise level, 30 nA) with a sample through-put of ca. 40–90 samples/h.
Keywords: carbon felt; electrochemical flow-biosensor; hydrogen peroxide; 2,6-dichlorophenol indophenol; horseradish peroxidase carbon felt; electrochemical flow-biosensor; hydrogen peroxide; 2,6-dichlorophenol indophenol; horseradish peroxidase
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Wang, Y.; Hasebe, Y. Carbon Felt-Based Bioelectrocatalytic Flow-Through Detectors: 2,6-Dichlorophenol Indophenol and Peroxidase Coadsorbed Carbon-Felt for Flow-Amperometric Determination of Hydrogen Peroxide. Materials 2014, 7, 1142-1154.

AMA Style

Wang Y, Hasebe Y. Carbon Felt-Based Bioelectrocatalytic Flow-Through Detectors: 2,6-Dichlorophenol Indophenol and Peroxidase Coadsorbed Carbon-Felt for Flow-Amperometric Determination of Hydrogen Peroxide. Materials. 2014; 7(2):1142-1154.

Chicago/Turabian Style

Wang, Yue; Hasebe, Yasushi. 2014. "Carbon Felt-Based Bioelectrocatalytic Flow-Through Detectors: 2,6-Dichlorophenol Indophenol and Peroxidase Coadsorbed Carbon-Felt for Flow-Amperometric Determination of Hydrogen Peroxide." Materials 7, no. 2: 1142-1154.


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