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Materials 2014, 7(2), 1142-1154; doi:10.3390/ma7021142
Article

Carbon Felt-Based Bioelectrocatalytic Flow-Through Detectors: 2,6-Dichlorophenol Indophenol and Peroxidase Coadsorbed Carbon-Felt for Flow-Amperometric Determination of Hydrogen Peroxide

1
 and
2,*
1 School of Chemical Engineering, University of Science and Technology Liaoning, 185 Qianshan Middle Road, High-tech zone, Anshan 114501, Liaoning, China 2 Department of Life Science and Green Chemistry, Faculty of Engineering, Saitama Institute of Technology, 1690 Fusaiji, Fukaya, Saitama 369-0293, Japan
* Author to whom correspondence should be addressed.
Received: 30 November 2013 / Revised: 8 January 2014 / Accepted: 7 February 2014 / Published: 12 February 2014
(This article belongs to the Special Issue Functional Materials and Proteins for Bio-Sensing Applications)
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Abstract

2,6-dichlorophenol indophenol (DCIP) and horseradish peroxidase (HRP) were coadsorbed on a porous carbon felt (CF) from their mixed aqueous solution under ultrasound irradiation for 5 min. The resulting DCIP and HRP-coadsorbed CF (DCIP/HRP-CF) showed an excellent bioelectrocatalytic activity for the reduction of H2O2. The coadsorption of DCIP together with HRP was essential to obtain larger bioelectrocatalytic current to H2O2. The DCIP/HRP-CF was successfully used as a working electrode unit of a bioelectrocatalytic flow-through detector for highly sensitive and continuous amperometric determination of H2O2. Under the optimized operational conditions (i.e., applied potential, +0.2 V versus Ag/AgCl; carrier pH 5.0, and carrier flow rate, 1.9 mL/min), the cathodic peak current of H2O2 linearly increased over the concentration range from 0.1 to 30 µM (the sensitivity, 0.88 µA/µM (slope of linear part); the limit of detection, 0.1 µM (S/N = 3) current noise level, 30 nA) with a sample through-put of ca. 40–90 samples/h.
Keywords: carbon felt; electrochemical flow-biosensor; hydrogen peroxide; 2,6-dichlorophenol indophenol; horseradish peroxidase carbon felt; electrochemical flow-biosensor; hydrogen peroxide; 2,6-dichlorophenol indophenol; horseradish peroxidase
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Wang, Y.; Hasebe, Y. Carbon Felt-Based Bioelectrocatalytic Flow-Through Detectors: 2,6-Dichlorophenol Indophenol and Peroxidase Coadsorbed Carbon-Felt for Flow-Amperometric Determination of Hydrogen Peroxide. Materials 2014, 7, 1142-1154.

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