Next Article in Journal
Silicate Removal in Aluminum Hydroxide Co-Precipitation Process
Next Article in Special Issue
Carbon Felt-Based Bioelectrocatalytic Flow-Through Detectors: 2,6-Dichlorophenol Indophenol and Peroxidase Coadsorbed Carbon-Felt for Flow-Amperometric Determination of Hydrogen Peroxide
Previous Article in Journal / Special Issue
Detection of Waterborne and Airborne Formaldehyde: From Amperometric Chemosensing to a Visual Biosensor Based on Alcohol Oxidase
Article Menu

Export Article

Open AccessArticle
Materials 2014, 7(2), 1069-1083; https://doi.org/10.3390/ma7021069

Direct Electrochemistry and Electrocatalysis of Horseradish Peroxidase Immobilized in a DNA/Chitosan-Fe3O4 Magnetic Nanoparticle Bio-Complex Film

School of Chemical Engineering, University of Science and Technology Liaoning, 185 Qianshan Road, Anshan 114051, Liaoning, China
*
Author to whom correspondence should be addressed.
Received: 30 November 2013 / Revised: 25 December 2013 / Accepted: 27 January 2014 / Published: 11 February 2014
(This article belongs to the Special Issue Functional Materials and Proteins for Bio-Sensing Applications)
Full-Text   |   PDF [1071 KB, uploaded 11 February 2014]

Abstract

A DNA/chitosan-Fe3O4 magnetic nanoparticle bio-complex film was constructed for the immobilization of horseradish peroxidase (HRP) on a glassy carbon electrode. HRP was simply mixed with DNA, chitosan and Fe3O4 nanoparticles, and then applied to the electrode surface to form an enzyme-incorporated polyion complex film. Scanning electron microscopy (SEM) was used to study the surface features of DNA/chitosan/Fe3O4/HRP layer. The results of electrochemical impedance spectroscopy (EIS) show that Fe3O4 and enzyme were successfully immobilized on the electrode surface by the DNA/chitosan bio-polyion complex membrane. Direct electron transfer (DET) and bioelectrocatalysis of HRP in the DNA/chitosan/Fe3O4 film were investigated by cyclic voltammetry (CV) and constant potential amperometry. The HRP-immobilized electrode was found to undergo DET and exhibited a fast electron transfer rate constant of 3.7 s−1. The CV results showed that the modified electrode gave rise to well-defined peaks in phosphate buffer, corresponding to the electrochemical redox reaction between HRP(Fe(III)) and HRP(Fe(II)). The obtained electrode also displayed an electrocatalytic reduction behavior towards H2O2. The resulting DNA/chitosan/Fe3O4/HRP/glassy carbon electrode (GCE) shows a high sensitivity (20.8 A·cm−2·M−1) toward H2O2. A linear response to H2O2 measurement was obtained over the range from 2 µM to 100 µM (R2 = 0.99) and an amperometric detection limit of 1 µM (S/N = 3). The apparent Michaelis-Menten constant of HRP immobilized on the electrode was 0.28 mM. Furthermore, the electrode exhibits both good operational stability and storage stability. View Full-Text
Keywords: horseradish peroxidase; direct electron transfer; DNA/chitosan polyion complex film; Fe3O4 magnetic nanoparticles; H2O2 biosensors horseradish peroxidase; direct electron transfer; DNA/chitosan polyion complex film; Fe3O4 magnetic nanoparticles; H2O2 biosensors
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Gu, T.; Wang, J.; Xia, H.; Wang, S.; Yu, X. Direct Electrochemistry and Electrocatalysis of Horseradish Peroxidase Immobilized in a DNA/Chitosan-Fe3O4 Magnetic Nanoparticle Bio-Complex Film. Materials 2014, 7, 1069-1083.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Materials EISSN 1996-1944 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top