Materials 2014, 7(2), 1055-1068; doi:10.3390/ma7021055
Article

Detection of Waterborne and Airborne Formaldehyde: From Amperometric Chemosensing to a Visual Biosensor Based on Alcohol Oxidase

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Received: 29 November 2013; in revised form: 17 December 2013 / Accepted: 8 January 2014 / Published: 11 February 2014
(This article belongs to the Special Issue Functional Materials and Proteins for Bio-Sensing Applications)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: A laboratory prototype of a microcomputer-based analyzer was developed for quantitative determination of formaldehyde in liquid samples, based on catalytic chemosensing elements. It was shown that selectivity for the target analyte could be increased by modulating the working electrode potential. Analytical parameters of three variants of the amperometric analyzer that differed in the chemical structure/configuration of the working electrode were studied. The constructed analyzer was tested on wastewater solutions that contained formaldehyde. A simple low-cost biosensor was developed for semi-quantitative detection of airborne formaldehyde in concentrations exceeding the threshold level. This biosensor is based on a change in the color of a solution that contains a mixture of alcohol oxidase from the yeast Hansenula polymorpha, horseradish peroxidase and a chromogen, following exposure to airborne formaldehyde. The solution is enclosed within a membrane device, which is permeable to formaldehyde vapors. The most efficient and sensitive biosensor for detecting formaldehyde was the one that contained alcohol oxidase with an activity of 1.2 U·mL−1. The biosensor requires no special instrumentation and enables rapid visual detection of airborne formaldehyde at concentrations, which are hazardous to human health.
Keywords: formaldehyde; microcomputer-based analyzer; chemosensing electrode; biosensor, alcohol oxidase; Hansenula polymorpha
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MDPI and ACS Style

Sigawi, S.; Smutok, O.; Demkiv, O.; Gayda, G.; Vus, B.; Nitzan, Y.; Gonchar, M.; Nisnevitch, M. Detection of Waterborne and Airborne Formaldehyde: From Amperometric Chemosensing to a Visual Biosensor Based on Alcohol Oxidase. Materials 2014, 7, 1055-1068.

AMA Style

Sigawi S, Smutok O, Demkiv O, Gayda G, Vus B, Nitzan Y, Gonchar M, Nisnevitch M. Detection of Waterborne and Airborne Formaldehyde: From Amperometric Chemosensing to a Visual Biosensor Based on Alcohol Oxidase. Materials. 2014; 7(2):1055-1068.

Chicago/Turabian Style

Sigawi, Sasi; Smutok, Oleh; Demkiv, Olha; Gayda, Galina; Vus, Bohdan; Nitzan, Yeshayahu; Gonchar, Mykhailo; Nisnevitch, Marina. 2014. "Detection of Waterborne and Airborne Formaldehyde: From Amperometric Chemosensing to a Visual Biosensor Based on Alcohol Oxidase." Materials 7, no. 2: 1055-1068.

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