Next Article in Journal / Special Issue
Detection of Waterborne and Airborne Formaldehyde: From Amperometric Chemosensing to a Visual Biosensor Based on Alcohol Oxidase
Previous Article in Journal
Nanocomposite Electrospun Nanofiber Membranes for Environmental Remediation
Previous Article in Special Issue
Long-Term Stability of a Cellulose-Based Glucose Oxidase Membrane
Article Menu

Export Article

Open AccessArticle
Materials 2014, 7(2), 1046-1054; doi:10.3390/ma7021046

Vascular Endothelial Growth Factor (VEGF) Detection Using an Aptamer and PNA-Based Bound/Free Separation System

Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 13 December 2013 / Revised: 15 January 2014 / Accepted: 28 January 2014 / Published: 11 February 2014
(This article belongs to the Special Issue Functional Materials and Proteins for Bio-Sensing Applications)
View Full-Text   |   Download PDF [330 KB, uploaded 11 February 2014]   |  

Abstract

We have developed a bound/free separation system using a vascular endothelial growth factor (VEGF) aptamer and a peptide nucleic acid (PNA) to detect VEGF. In this system, we designed capture PNA (CaPNA), which hybridizes with the aptamer in the absence of the target protein, but does not hybridize with the aptamer in the presence of the target protein due to steric hindrance and/or stabilization of the aptamer’s structure. By removing the aptamers not bound to the target protein using CaPNA immobilized beads, we can detect the target protein by measuring signals labeled with the aptamer in the supernatant. In this study, we detected VEGF using CaPNA-immobilized beads without the time-consuming washing step. This simple and rapid system can detect 25 nM of VEGF in 15 min.
Keywords: aptamer; bound free separation; peptide nucleic acid aptamer; bound free separation; peptide nucleic acid
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Mita, C.; Abe, K.; Fukaya, T.; Ikebukuro, K. Vascular Endothelial Growth Factor (VEGF) Detection Using an Aptamer and PNA-Based Bound/Free Separation System. Materials 2014, 7, 1046-1054.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Materials EISSN 1996-1944 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top