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Materials 2013, 6(2), 669-681; doi:10.3390/ma6020669
Article

Supported Membranes Meet Flat Fluidics: Monitoring Dynamic Cell Adhesion on Pump-Free Microfluidics Chips Functionalized with Supported Membranes Displaying Mannose Domains

1,2,†
, 1,3,†
, 1,4
, 5
, 2,*  and 1,3,6,*
Received: 18 October 2012; in revised form: 7 January 2013 / Accepted: 5 February 2013 / Published: 22 February 2013
(This article belongs to the Special Issue Supported Lipid Membranes)
Download PDF [1528 KB, uploaded 22 February 2013]
Abstract: In this paper we demonstrate the combination of supported membranes and so-called flat microfluidics, which enables one to manipulate liquids on flat chip surfaces via “inverse piezoelectric effect”. Here, an alternating external electric field applied to the inter-digital transducers excites a surface acoustic wave on a piezoelectric substrate. Employing lithographic patterning of self-assembled monolayers of alkoxysilanes, we successfully confine a free-standing, hemi-cylindrical channel with the volume of merely 7 µL . The experimentally determined maximum flow velocity scales linearly with the acoustic power, suggesting that our current setup can drive liquids at the speed of up to 7 cm/s (corresponding to a shear rate of 280 s−1) without applying high pressures using a fluidic pump. After the establishment of the functionalization of fluidic chip surfaces with supported membranes, we deposited asymmetric supported membranes displaying well-defined mannose domains and monitored the dynamic adhesion of E. Coli HB101 expressing mannose-binding receptors. Despite of the further technical optimization required for the quantitative analysis, the obtained results demonstrate that the combination of supported membranes and flat fluidics opens a large potential to investigate dynamic adhesion of cells on biofunctional membrane surfaces with the minimum amount of samples, without any fluidic pump.
Keywords: supported membrane; surface acoustic wave; flatfluidics; cell adhesion supported membrane; surface acoustic wave; flatfluidics; cell adhesion
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Oelke, J.; Kaindl, T.; Pasc, A.; Guttenberg, Z.; Wixforth, A.; Tanaka, M. Supported Membranes Meet Flat Fluidics: Monitoring Dynamic Cell Adhesion on Pump-Free Microfluidics Chips Functionalized with Supported Membranes Displaying Mannose Domains. Materials 2013, 6, 669-681.

AMA Style

Oelke J, Kaindl T, Pasc A, Guttenberg Z, Wixforth A, Tanaka M. Supported Membranes Meet Flat Fluidics: Monitoring Dynamic Cell Adhesion on Pump-Free Microfluidics Chips Functionalized with Supported Membranes Displaying Mannose Domains. Materials. 2013; 6(2):669-681.

Chicago/Turabian Style

Oelke, Jochen; Kaindl, Thomas; Pasc, Andreea; Guttenberg, Zeno; Wixforth, Achim; Tanaka, Motomu. 2013. "Supported Membranes Meet Flat Fluidics: Monitoring Dynamic Cell Adhesion on Pump-Free Microfluidics Chips Functionalized with Supported Membranes Displaying Mannose Domains." Materials 6, no. 2: 669-681.


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