Next Article in Journal
Utilization of an Ionic Liquid/Urea Mixture as a Physical Coupling Agent for Agarose/Talc Composite Films
Next Article in Special Issue
Dynamic Morphological Changes Induced By GM1 and Protein Interactions on the Surface of Cell-Sized Liposomes
Previous Article in Journal
Advanced Strategies for Articular Cartilage Defect Repair
Previous Article in Special Issue
Horizontal Bilayer for Electrical and Optical Recordings
Article Menu

Export Article

Open AccessArticle
Materials 2013, 6(2), 669-681; doi:10.3390/ma6020669

Supported Membranes Meet Flat Fluidics: Monitoring Dynamic Cell Adhesion on Pump-Free Microfluidics Chips Functionalized with Supported Membranes Displaying Mannose Domains

Department of Physics, Technical University Munich, Garching D85748, Germany
Experimental Physics I, University of Augsburg, Augsburg D86159, Germany
Physical Chemistry of Biosystems, University of Heidelberg, Heidelberg D69120, Germany
NANO group-UMR 7565 SRSMC CNRS, Université de Lorraine, Boulevard des Aiguillettes-F54506 Vandoeuvre-Lès-Nancy, France
Beckman Coulter Biomedical GmbH, Advalytix Products, Munich D81377, Germany
Cell Biophysics Lab, Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Karlsruhe D76131, Germany
These two authors equally contributed to the work.
Authors to whom correspondence should be addressed.
Received: 18 October 2012 / Revised: 7 January 2013 / Accepted: 5 February 2013 / Published: 22 February 2013
(This article belongs to the Special Issue Supported Lipid Membranes)
View Full-Text   |   Download PDF [1528 KB, uploaded 22 February 2013]   |  


In this paper we demonstrate the combination of supported membranes and so-called flat microfluidics, which enables one to manipulate liquids on flat chip surfaces via “inverse piezoelectric effect”. Here, an alternating external electric field applied to the inter-digital transducers excites a surface acoustic wave on a piezoelectric substrate. Employing lithographic patterning of self-assembled monolayers of alkoxysilanes, we successfully confine a free-standing, hemi-cylindrical channel with the volume of merely 7 µL . The experimentally determined maximum flow velocity scales linearly with the acoustic power, suggesting that our current setup can drive liquids at the speed of up to 7 cm/s (corresponding to a shear rate of 280 s−1) without applying high pressures using a fluidic pump. After the establishment of the functionalization of fluidic chip surfaces with supported membranes, we deposited asymmetric supported membranes displaying well-defined mannose domains and monitored the dynamic adhesion of E. Coli HB101 expressing mannose-binding receptors. Despite of the further technical optimization required for the quantitative analysis, the obtained results demonstrate that the combination of supported membranes and flat fluidics opens a large potential to investigate dynamic adhesion of cells on biofunctional membrane surfaces with the minimum amount of samples, without any fluidic pump. View Full-Text
Keywords: supported membrane; surface acoustic wave; flatfluidics; cell adhesion supported membrane; surface acoustic wave; flatfluidics; cell adhesion

Figure 1

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Oelke, J.; Kaindl, T.; Pasc, A.; Guttenberg, Z.; Wixforth, A.; Tanaka, M. Supported Membranes Meet Flat Fluidics: Monitoring Dynamic Cell Adhesion on Pump-Free Microfluidics Chips Functionalized with Supported Membranes Displaying Mannose Domains. Materials 2013, 6, 669-681.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Materials EISSN 1996-1944 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top