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• Oelke, J
• Kaindl, T
• Pasc, A
• Guttenberg, Z
• Wixforth, A
• Tanaka, M
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• Oelke, J
• Kaindl, T
• Pasc, A
• Guttenberg, Z
• Wixforth, A
• Tanaka, M
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• Oelke, J
• Kaindl, T
• Pasc, A
• Guttenberg, Z
• Wixforth, A
• Tanaka, M

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Materials 2013, 6(2), 669-681; doi:10.3390/ma6020669

Article

Supported Membranes Meet Flat Fluidics: Monitoring Dynamic Cell Adhesion on Pump-Free Microfluidics Chips Functionalized with Supported Membranes Displaying Mannose Domains

Jochen Oelke ^1,2,† , Thomas Kaindl ^1,3,† , Andreea Pasc ^1,4 , Zeno Guttenberg ⁵ , Achim Wixforth ^2,*  and Motomu Tanaka ^1,3,6,*

¹ Department of Physics, Technical University Munich, Garching D85748, Germany ² Experimental Physics I, University of Augsburg, Augsburg D86159, Germany ³ Physical Chemistry of Biosystems, University of Heidelberg, Heidelberg D69120, Germany ⁴ NANO group-UMR 7565 SRSMC CNRS, Université de Lorraine, Boulevard des Aiguillettes-F54506 Vandoeuvre-Lès-Nancy, France ⁵ Beckman Coulter Biomedical GmbH, Advalytix Products, Munich D81377, Germany ⁶ Cell Biophysics Lab, Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Karlsruhe D76131, Germany ^† These two authors equally contributed to the work.

* Authors to whom correspondence should be addressed.

Received: 18 October 2012; in revised form: 7 January 2013 / Accepted: 5 February 2013 / Published: 22 February 2013

(This article belongs to the Special Issue Supported Lipid Membranes)

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Abstract: In this paper we demonstrate the combination of supported membranes and so-called flat microfluidics, which enables one to manipulate liquids on flat chip surfaces via “inverse piezoelectric effect”. Here, an alternating external electric field applied to the inter-digital transducers excites a surface acoustic wave on a piezoelectric substrate. Employing lithographic patterning of self-assembled monolayers of alkoxysilanes, we successfully confine a free-standing, hemi-cylindrical channel with the volume of merely 7 µL . The experimentally determined maximum flow velocity scales linearly with the acoustic power, suggesting that our current setup can drive liquids at the speed of up to 7 cm/s (corresponding to a shear rate of 280 s⁻¹) without applying high pressures using a fluidic pump. After the establishment of the functionalization of fluidic chip surfaces with supported membranes, we deposited asymmetric supported membranes displaying well-defined mannose domains and monitored the dynamic adhesion of E. Coli HB101 expressing mannose-binding receptors. Despite of the further technical optimization required for the quantitative analysis, the obtained results demonstrate that the combination of supported membranes and flat fluidics opens a large potential to investigate dynamic adhesion of cells on biofunctional membrane surfaces with the minimum amount of samples, without any fluidic pump.

Keywords: supported membrane; surface acoustic wave; flatfluidics; cell adhesion

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