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Horizontal Bilayer for Electrical and Optical Recordings
Biophysics, Department of Biology/Chemistry, University Osnabrueck, Barbarastr. 13, Osnabrueck 49076, Germany
Ionovation GmbH, Westerbreite 7, Osnabrueck 49084, Germany
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Faßberg 11, Goettingen 37077, Germany
* Author to whom correspondence should be addressed.
Received: 15 October 2012; in revised form: 19 November 2012 / Accepted: 4 December 2012 / Published: 10 December 2012
Abstract: Artificial bilayer containing reconstituted ion channels, transporters and pumps serve as a well-defined model system for electrophysiological investigations of membrane protein structure–function relationship. Appropriately constructed microchips containing horizontally oriented bilayers with easy solution access to both sides provide, in addition, the possibility to investigate these model bilayer membranes and the membrane proteins therein with high resolution fluorescence techniques up to the single-molecule level. Here, we describe a bilayer microchip system in which long-term stable horizontal free-standing and hydrogel-supported bilayers can be formed and demonstrate its prospects particularly for single-molecule fluorescence spectroscopy and high resolution fluorescence microscopy in probing the physicochemical properties like phase behavior of the bilayer-forming lipids, as well as in functional studies of membrane proteins.
Keywords: horizontal bilayer; simultaneous electrical-optical recordings; membrane dynamics; lipid diffusion; supported bilayer
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MDPI and ACS Style
Bartsch, P.; Walter, C.; Selenschik, P.; Honigmann, A.; Wagner, R. Horizontal Bilayer for Electrical and Optical Recordings. Materials 2012, 5, 2705-2730.
Bartsch P, Walter C, Selenschik P, Honigmann A, Wagner R. Horizontal Bilayer for Electrical and Optical Recordings. Materials. 2012; 5(12):2705-2730.
Bartsch, Philipp; Walter, Claudius; Selenschik, Philipp; Honigmann, Alf; Wagner, Richard. 2012. "Horizontal Bilayer for Electrical and Optical Recordings." Materials 5, no. 12: 2705-2730.